製品の概要

  • 製品名

  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell culture supernatant, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 100 pg/ml
  • 検出範囲

    123 pg/ml - 30000 pg/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 99.29 89% - 111%
    Plasma 92.63 78% - 104%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Rat
  • 製品の概要

    Abcam’s IFN gamma Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IFN gamma in plasma and cell culture supernatants. (Rat IFN gamma concentration in normal plasma is pretty low, it may not be detected in this assay).


    This assay employs an antibody specific for IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat IFN-gamma in normal serum with ab113349, only in serum spiked with Rat IFN-gamma.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat IFN Gamma (lyophilized) 2 vials
    IFN gamma Microplate (12 x 8 wells) 1 x 96 units
    Recombinant rat IFN gamma Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 組織特異性

    Released primarily from activated T lymphocytes.
  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 配列類似性

    Belongs to the type II (or gamma) interferon family.
  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab113349

  • Representative standard curve using ab113349

プロトコール

参考文献

This product has been referenced in:

  • Sun Y  et al. Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension. Cell Physiol Biochem 44:1715-1725 (2017). Read more (PubMed: 29216636) »
See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab113349.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録