Rat GPNMB ELISA Kit (ab270219)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 136 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat GPNMB ELISA Kit
GPNMB キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 7 4.3% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
136 pg/ml -
検出範囲
0.31 ng/ml - 20 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 104 94% - 122% Serum 98 95% - 101% Cell culture media 112 109% - 114% Hep Plasma 105 94% - 121% EDTA Plasma 102 97% - 112% Cit plasma 120 112% - 133% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat -
製品の概要
Rat GPNMB ELISA Kit (ab270219) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in cell culture media, cell culture supernatant, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat GPNMB with 136 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. The GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat GPNMB Capture Antibody 1 x 600µl 10X Rat GPNMB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat GPNMB Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Could be a melanogenic enzyme. -
組織特異性
Not restricted to the melanocytic lineage. -
配列類似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
発生段階
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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別名
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
参照データベース
- Entrez Gene: 113955 Rat
- SwissProt: Q6P7C7 Rat
- Unigene: 13778 Rat
関連製品
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Alternative Versions
画像
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The GPNMB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (EDTA) 40%, plasma (heparin) 20% and spleen supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 59 ng/mL in neat serum, 48 ng/mL in neat plasma (citrate), 41 ng/mL in neat plasma (EDTA), 69 ng/mL in neat plasma (heparin) and 24 ng/mL in neat spleen supernatant.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab270219 は論文での使用が確認できていません。