Rat Cystatin C ELISA Kit (ab201281)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.72 pg/ml
- Range: 23.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat Cystatin C ELISA Kit
Cystatin C キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Cystatin C 8 2.95% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Cystatin C 3 3.52% -
サンプルの種類
Cell culture supernatant, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
11.72 pg/ml -
検出範囲
23.4 pg/ml - 1500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 113 111% - 114% Cell culture media 110 107% - 116% EDTA Plasma 115 108% - 119% Cit plasma 112 109% - 116% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat
非交差種: Cow, Pig -
製品の概要
Rat Cystatin C ELISA Kit (ab201281) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Cystatin C with 11.72 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat Cystatin C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CP 1 x 6ml Plate Seals 1 unit Rat Cystatin C Capture Antibody (lyophilized) 1 vial Rat Cystatin C Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
組織特異性
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
関連疾患
Amyloidosis 6
Macular degeneration, age-related, 11 -
配列類似性
Belongs to the cystatin family. -
翻訳後修飾
The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
see all -
参照データベース
- Entrez Gene: 25307 Rat
- SwissProt: P14841 Rat
- Unigene: 106351 Rat
画像
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Standard curve comparison between rat Cystatin C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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The 1X dilution was 0.2% for serum, and 0.4% for citrate and EDTA plasma. Interpolated data values (mean +/- SD, n = 2) are graphed.
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The 1X dilution for the brain extract was an extract load of 15.6 µg/mL, and a load of 125 µg/mL for the heart extract.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab201281 は 8 報の論文で使用されています。
- Tekin Neijmann Ş et al. Evaluation of renal function in rats with moderate and mild brain trauma. Ulus Travma Acil Cerrahi Derg 28:1-7 (2022). PubMed: 34967428
- Miyasaki DM et al. Treatment of Chronic Kidney Disease with Extracellular Vesicles from Mesenchymal Stem Cells and CD133+ Expanded Cells: A Comparative Preclinical Analysis. Int J Mol Sci 23:N/A (2022). PubMed: 35269664
- Zeinali H et al. Reduced inflammation following human endometrial stromal/stem cell injection into male Wistar rats with cisplatin-induced acute kidney injury. Bioimpacts 12:439-448 (2022). PubMed: 36381637
- Idris AO et al. Evaluation of acrylamide exposure in pregnant Wistar rats as a risk of developing renal disease in their litters. Environ Sci Pollut Res Int 28:39680-39691 (2021). PubMed: 33763836
- El-Sonbaty SM et al. Amphora algae with low-level ionizing radiation exposure ameliorate D-galactosamine-induced inflammatory impairment in rat kidney. Environ Toxicol 36:451-459 (2021). PubMed: 33107697
- Salem HR & Faried MA Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats. ScientificWorldJournal 2021:1393372 (2021). PubMed: 34887703
- Çakir M et al. Protective effect of N-(p-amylcinnamoyl) anthranilic acid, phospholipase A2 enzyme inhibitor, and transient receptor potential melastatin-2 channel blocker against renal ischemia-reperfusion injury. J Cell Biochem 120:3822-3832 (2019). PubMed: 30259992
- Drummond CA et al. Cigarette smoking causes epigenetic changes associated with cardiorenal fibrosis. Physiol Genomics 48:950-960 (2016). PubMed: 27789733