Rat CXCL10 ELISA Kit (ab270896)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 87.45 pg/ml
- Range: 117.18 pg/ml - 7500 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat CXCL10 ELISA Kit
IP10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Supernatant 8 4.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Supernatant 3 6.7% -
サンプルの種類
Cell culture supernatant -
アッセイタイプ
Sandwich (quantitative) -
検出感度
87.45 pg/ml -
検出範囲
117.18 pg/ml - 7500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96 85% - 104% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat -
製品の概要
Rat CXCL10 ELISA kit (ab270896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL10 protein in rat cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate rat CXCL10 with 87.45 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Rat C-X-C motif chemokine 10 (CXCL10) is a 10 kDa protein that is secreted by several cell types in response to interferon-gamma. These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3). Rat CXCL10 shares a 79% and 68% sequence identity with mouse and human, respectively.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat CXCL10 Capture Antibody 1 x 600µl 10X Rat CXCL10 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat CXCL10 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
参照データベース
- Entrez Gene: 245920 Rat
- SwissProt: P48973 Rat
- Unigene: 10584 Rat
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CXCL10 standard curve in Sample Diluent NS.The CXCL10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL10 in rat tissue culture supernatant samples.The concentrations of CXCL10 were measured in duplicates, interpolated from the CXCL10 standard curves and corrected for sample dilution. Undiluted samples are as follows: 5 ug/mL LPS stimulated spleen culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL10 concentration was determined to be 6364.20 pg/mL in and stimulated spleen supernatant.
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Sandwich ELISA - Rat CXCL10 ELISA Kit (ab270896)To learn more about the advantages of recombinant antibodies see here.
参考文献 (0)
ab270896 は論文での使用が確認できていません。
