Rat CD62L ELISA Kit (Selectin L) (ab100776)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat CD62L ELISA Kit (Selectin L)
CD62L キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
8.23 pg/ml - 6000 pg/ml -
添加回収試験
96 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 98.42 89% - 107% Serum 97.06 86% - 107% Plasma 94.33 85% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s CD62L Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD62L in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CD62L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD62L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti Rat CD62L 2 vials CD62L Microplate (12 x 8 wells) 1 unit Recombinant rat CD62L Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
細胞内局在
Cell Membrane; single-pass type I membrane protein. -
別名
- A.11
- AI528707
- CD62 antigen ligand
see all -
参照データベース
- Entrez Gene: 29259 Rat
- SwissProt: Q63762 Rat
画像
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Typical standard curve
Representative standard curve using ab100776
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Typical standard curveRepresentative standard curve using ab100776
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100776 は論文での使用が確認できていません。