Rat alpha 2 Macroglobulin ELISA Kit (ab157730)
Key features and details
- Sensitivity: 2.23 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat alpha 2 Macroglobulin ELISA Kit
alpha 2 Macroglobulin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma, Other biological fluids -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.23 ng/ml -
検出範囲
6.25 ng/ml - 400 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s alpha 2 Macroglobulin Rat ELISA Kit is highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of alpha 2 Macroglobulin in rat biological samples. This kit is specific to the alpha 2 Macroglobulin and will not cross react with the closely related alpha 1 Macroglobulin.
In this assay the alpha 2 Macroglobulin present in samples reacts with the anti-alpha 2 Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-alpha 2 Macroglobulin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 2 Macroglobulin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 2 Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 2 Macroglobulin in the test sample. The quantity of alpha 2 Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-rat alpha 2 Macroglobulin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat alpha 2 Macroglobulin Calibrator (lyophilized) 1 vial Rat alpha 2 Macroglobulin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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機能
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
組織特異性
Secreted in plasma. -
配列類似性
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
発生段階
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
細胞内局在
Secreted. - Information by UniProt
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別名
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
参照データベース
- Entrez Gene: 24153 Rat
- SwissProt: P06238 Rat
- Unigene: 225884 Rat
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab157730 は 2 報の論文で使用されています。
- Fan Y et al. Longitudinal Model of Periprosthetic Joint Infection in the Rat. J Orthop Res 38:1101-1112 (2020). PubMed: 31808572
- Maldonado-Bouchard S et al. Inflammation is increased with anxiety- and depression-like signs in a rat model of spinal cord injury. Brain Behav Immun 51:176-95 (2016). PubMed: 26296565