Rat Adiponectin ELISA Kit (ab108784)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat Adiponectin ELISA Kit
Adiponectin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 7.2% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 1.5 ng/ml -
検出範囲
1.56 ng/ml - 100 ng/ml -
添加回収試験
98 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s Adiponectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Adiponectin in urine, plasma, serum, and cell culture supernatants.
An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Adiponectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adiponectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat Adiponectin Antibody 1 x 120µl Adiponectin Microplate (12 x 8 well strips) 1 unit Adiponectin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
組織特異性
Synthesized exclusively by adipocytes and secreted into plasma. -
関連疾患
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
ドメイン
The C1q domain is commonly called the globular domain. -
翻訳後修飾
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
細胞内局在
Secreted. - Information by UniProt
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別名
- 30 kDa adipocyte complement related protein
- 30 kDa adipocyte complement-related protein
- ACDC
see all -
参照データベース
画像
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Sandwich ELISA - ab108784 Adiponectin Rat ELISA kit
Adiponectin measured in tissue lysates showing quantity (ng) per 1 million cells
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Sandwich ELISA - ab108784 Adiponectin Rat ELISA kit -
Typical Standard CurveRepresentative Standard Curve using ab108784.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab108784 は 9 報の論文で使用されています。
- Lin YJ et al. Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing. Placenta 78:44-53 (2019). PubMed: 30955710
- Dobrocsyova V et al. Modulation of Adipogenesis and Oxidative Status by Quercetin and Ochratoxin A: Positive or Negative Impact on Rat Adipocyte Metabolism? Molecules 24:N/A (2019). PubMed: 31623151
- Honoré SM et al. Smallanthus sonchifolius (Yacon) Flour Improves Visceral Adiposity and Metabolic Parameters in High-Fat-Diet-Fed Rats. J Obes 2018:5341384 (2018). PubMed: 30510798
- Liu X et al. Chinese herb extract improves liver steatosis by promoting the expression of high molecular weight adiponectin in NAFLD rats. Mol Med Rep 16:5580-5586 (2017). PubMed: 28849192
- Panetta P et al. Long-Term Sex-Dependent Vulnerability to Metabolic challenges in Prenatally Stressed Rats. Front Behav Neurosci 11:113 (2017). PubMed: 28706476
- Zhao L et al. Globular adiponectin ameliorates metabolic insulin resistance via AMPK-mediated restoration of microvascular insulin responses. J Physiol 593:4067-79 (2015). PubMed: 26108677
- Youcef G et al. Simultaneous characterization of metabolic, cardiac, vascular and renal phenotypes of lean and obese SHHF rats. PLoS One 9:e96452 (2014). PubMed: 24831821
- Hajje G et al. Hypothyroidism and its rapid correction alter cardiac remodeling. PLoS One 9:e109753 (2014). Sandwich ELISA ; Rat . PubMed: 25333636
- Zhao L et al. Globular adiponectin enhances muscle insulin action via microvascular recruitment and increased insulin delivery. Circ Res 112:1263-71 (2013). PubMed: 23459195