Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7127] to RASSF1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
製品名Anti-RASSF1 antibody [EPR7127] - BSA and Azide free
RASSF1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7127] to RASSF1 - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC,ICC/IF or IP
Synthetic peptide within Human RASSF1 aa 300-400. The exact sequence is proprietary.
Database link: Q9NS23
ab248174 is the carrier-free version of ab126764 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248174 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 7.50 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
機能Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
組織特異性Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
配列類似性Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-associating domain.
Contains 1 SARAH domain.
細胞内局在Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
- Information by UniProt
- 123F2 antibody
- Cardiac specific ras association domain family 1 protein antibody
- cardiac-specific ras association domain family 1 protein, 123F2 antibody
All lanes : Anti-RASSF1 antibody [EPR7127] (ab126764) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
This data was developed using ab126764, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.