Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133) - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
製品名Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] - BSA and Azide free
RASGRP3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133) - BSA and Azide free
This antibody only detects RASGRP3 phosphorylated at Threonine 133.
アプリケーション適用あり: ICC/IF, WBmore details
適用なし: IHC-P or IP
種交差性交差種: Mouse, Human
Synthetic peptide within Human RASGRP3 (phospho T133). The exact sequence is proprietary.
ab248022 is the carrier-free version of ab124823 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.|
機能Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
配列類似性Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
- Information by UniProt
- Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
- Grp3 antibody
- GRP3_HUMAN antibody
All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution
Lane 1 : Ramos cell lysate, untreated
Lane 2 : Ramos cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa
This data was developed using ab124823, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.