Anti-RASGRP3 (phospho T133) 抗体 [EPNCIR148] - BSA and Azide free (ab248022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] - BSA and Azide free
RASGRP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPNCIR148] to RASGRP3 (phospho T133) - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse非交差種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248022 is the carrier-free version of ab124823.
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPNCIR148 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248022の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa. |
ターゲット情報
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機能
Guanine nucleotide exchange factor (GEF) for Ras and Rap1. -
配列類似性
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain. - Information by UniProt
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参照データベース
- Entrez Gene: 25780 Human
- Entrez Gene: 240168 Mouse
- Omim: 609531 Human
- SwissProt: Q8IV61 Human
- Unigene: 143674 Human
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別名
- Calcium and DAG-regulated guanine nucleotide exchange factor III antibody
- Grp3 antibody
- GRP3_HUMAN antibody
see all
画像
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All lanes : Anti-RASGRP3 (phospho T133) antibody [EPNCIR148] (ab124823) at 1/1000 dilution
Lane 1 : Ramos cell lysate, untreated
Lane 2 : Ramos cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDaThis data was developed using ab124823, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248022 は論文での使用が確認できていません。