Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain Organoid)
Permeabilization
Yes - 0.1%TX
Specification
Brain Organoid
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 488 Donkey Anti-Mouse IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Also tested 0.3%TX permeablization
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 27 2020