Anti-RASA1 抗体 [B4F8] (ab2922)

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Key features and details

  • Mouse monoclonal [B4F8] to RASA1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-RASA1 antibody [B4F8]
    RASA1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [B4F8] to RASA1

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for RASA1 (an alternate name of GAP).

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Non human primates

  • 免疫原

    Recombinant full length protein corresponding to Human RASA1.

  • エピトープ

    Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.

  • ポジティブ･コントロール

    • RS-2 cell lysate. Mouse fibroblast cells.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 一次抗体 備考

    GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.

  • ポリ/モノ

    モノクローナル

  • クローン名

    B4F8

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.

Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.
WB Use a concentration of 10 µg/ml.

By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate.

ターゲット情報

  • 機能

    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

  • 組織特異性

    In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

  • 関連疾患

    Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
    Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
    Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.

  • 配列類似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • 翻訳後修飾

    The N-terminus is blocked.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CM AVM antibody
    • CMAVM antibody
    • DKFZp434N071 antibody
    • GAP antibody
    • GTPase activating protein antibody
    • GTPase-activating protein antibody
    • OTTHUMP00000222390 antibody
    • OTTHUMP00000222391 antibody
    • OTTHUMP00000222392 antibody
    • OTTHUMP00000222393 antibody
    • p120GAP antibody
    • p120RASGAP antibody
    • PKWS antibody
    • Ras GTPase-activating protein 1 antibody
    • RAS p21 protein activator (GTPase activating protein) 1 antibody
    • Ras p21 protein activator antibody
    • RASA antibody
    • RASA1 antibody
    • RASA1_HUMAN antibody
    • RasGAP antibody
    • Triphosphatase activating protein antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
    Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

    ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
    Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
    All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse brain cell lysate

    Lysates/proteins at 25 µg per lane.

    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

参考文献 (3)

ab2922 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2922 は 3 報の論文で使用されています。

  • Lei Z  et al. MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway. J Cell Mol Med 19:1994-2005 (2015). WB, IF . PubMed: 25945589
  • Fiedler J  et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . PubMed: 21788589
  • Settleman J  et al. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell 69:539-49 (1992). PubMed: 1581965

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