Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution
1/100
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP donkey anti-mouse
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane 1 - HeLa cells
Lane 2 - MEF cells
This antibody does not detect any bands in a denaturing Western blot. It is possible it only detects native, folded protein.
Abcam response
Please note that this antibody is not recommended for Western Blot
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 26 2021