Application
Western blot
Sample
Human Cell lysate - whole cell (PANC-1)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
PANC-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution
1/50
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: HRP donkey anti-mouse
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
This antibody does not detect any bands in a denaturing Western blot. It is possible it only detects native, folded protein.
Lane 1: PANC-1 cells
Lane 2: RASless MEF cells overexpressing BRAF
Lane 3: RASless MEF cells overexpressing HRAS
Lane 4: RASless MEF cells overexpressing NRAS
Lane 5: RASless MEF cells overexpressing KRAS
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 08 2021