Anti-Ras 抗体 [F132-62] (ab16907)
![Flow Cytometry - Anti-Ras antibody [F132-62] (ab16907)](https://www.abcam.co.jp/ps/products/16/ab16907/Images/ab16907-25791-anti-ras-antibody-f132-62-flow-cytometry.jpg "Flow Cytometry - Anti-Ras antibody [F132-62] (ab16907)")
Key features and details
- Mouse monoclonal [F132-62] to Ras
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ras antibody [F132-62]
Ras 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [F132-62] to Ras -
由来種
Mouse -
特異性
Reactive with the Ras translational products of the H-, K- and N-ras human, as well as mouse and rat c- and v-ras oncogenes. We have received mixed results for Western Blot. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human).
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ポジティブ･コントロール
- Sw480 or Y1 cells or normal skin tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.2% Gelatin, 0.82% Sodium phosphate -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
F132-62 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16907の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
関連疾患
Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC). -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
翻訳後修飾
Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. -
細胞内局在
Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 3265 Human
- Entrez Gene: 3845 Human
- Entrez Gene: 4893 Human
- Omim: 164790 Human
- Omim: 190020 Human
- Omim: 190070 Human
- SwissProt: P01111 Human
- SwissProt: P01112 Human
see all -
別名
- C BAS/HAS antibody
- C HA RAS1 antibody
- C-BAS/HAS antibody
see all
画像
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Flow Cytometry - Anti-Ras antibody [F132-62] (ab16907)
Overlay histogram showing HeLa cells stained with ab16907 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16907, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab16907 は 12 報の論文で使用されています。
- Wang H et al. Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway. Aging (Albany NY) 12:16021-16034 (2020). PubMed: 32756011
- Wang S et al. Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. BMC Cancer 19:1160 (2019). PubMed: 31783811
- Zeng M et al. Chinese yam extract and adenosine attenuated LPS-induced cardiac dysfunction by inhibiting RAS and apoptosis via the ER-mediated activation of SHC/Ras/Raf1 pathway. Phytomedicine 61:152857 (2019). PubMed: 31035040
- Xue C et al. Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice. Cell Prolif 52:e12557 (2019). PubMed: 30485569
- Choi BH et al. RAS GTPases are modified by SUMOylation. Oncotarget 9:4440-4450 (2018). PubMed: 29435114