Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6298] to RARS - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-RARS antibody [EPR6298] - BSA and Azide free
RARS 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6298] to RARS - BSA and Azide free
アプリケーション適用あり: ICC/IF, Flow Cyt, WBmore details
適用なし: IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human RARS aa 600-700. The exact sequence is proprietary.
ab248245 is the carrier-free version of ab128956 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248245 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Arginyl tRNA synthetase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 4.20 x 10 -12 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60-72 kDa (predicted molecular weight: 75 kDa).|
機能Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
配列類似性Belongs to the class-I aminoacyl-tRNA synthetase family.
ドメインThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
- Information by UniProt
製品の状態There are 2 isoforms produced by alternative initiation.
- 2610011N19Rik antibody
- 2610037E21Rik antibody
- AL033339 antibody
All lanes : Anti-RARS antibody [EPR6298] (ab128956) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 75 kDa
Observed band size: 60-72 kDa why is the actual band size different from the predicted?
This data was developed using ab128956, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.