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Anti-RAP1 抗体 [4c8/1] (ab14404)

Reviews (4)Q&A (1)References (2)
Western blot - Anti-RAP1 antibody [4c8/1] (ab14404)
  • Immunoprecipitation - Anti-RAP1 antibody [4c8/1] (ab14404)
  • Flow Cytometry - Anti-RAP1 antibody [4c8/1] (ab14404)

Key features and details

  • Mouse monoclonal [4c8/1] to RAP1
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-RAP1 antibody [4c8/1]
    RAP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4c8/1] to RAP1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IP, WB, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Human Rap1 protein.

  • ポジティブ･コントロール

    • In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; A431.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14404の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use a concentration of 5 µg/ml.
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab91366 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
特記事項
IP
Use a concentration of 5 µg/ml.
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab91366 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.

  • 組織特異性

    Ubiquitous. Highly expressed.

  • 配列類似性

    Belongs to the RAP1 family.
    Contains 1 BRCT domain.
    Contains 1 Myb-like domain.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Dopamine receptor interacting protein 5 antibody
      • Dopamine receptor-interacting protein 5 antibody
      • DRIP 5 antibody
      • DRIP5 antibody
      • hRap1 antibody
      • MGC105533 antibody
      • RAP 1 antibody
      • RAP1 homolog antibody
      • RAP1, yeast, homolog if antibody
      • RAP1, yeast, homolog of antibody
      • Repressor/activator protein 1 homolog antibody
      • TE2IP_HUMAN antibody
      • Telomeric Repeat Binding Factor 2 Interacting Protein antibody
      • Telomeric repeat-binding factor 2-interacting protein 1 antibody
      • TERF2-interacting protein antibody
      • TERF2-interacting telomeric protein 1 antibody
      • TERF2IP antibody
      • TRF2 Interacting Telomeric Protein RAP1 antibody
      • TRF2 interacting telomeric RAP1 protein antibody
      • TRF2-interacting telomeric protein 1 antibody
      • TRF2-interacting telomeric protein antibody
      see all

    画像

    • Western blot - Anti-RAP1 antibody [4c8/1] (ab14404)
      Western blot - Anti-RAP1 antibody [4c8/1] (ab14404)
      All lanes : Anti-RAP1 antibody [4c8/1] (ab14404) at 5 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes
    • Immunoprecipitation - Anti-RAP1 antibody [4c8/1] (ab14404)
      Immunoprecipitation - Anti-RAP1 antibody [4c8/1] (ab14404)
      RAP1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to RAP1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14404.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 55kDa; RAP1
    • Flow Cytometry - Anti-RAP1 antibody [4c8/1] (ab14404)
      Flow Cytometry - Anti-RAP1 antibody [4c8/1] (ab14404)
      Overlay histogram showing HeLa cells stained with ab14404 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14404, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献 (2)

    ab14404 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14404 は 2 報の論文で使用されています。

    • Pereira CD  et al. Nuclear Accumulation of LAP1:TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells 9:N/A (2020). PubMed: 32751253
    • Wang J  et al. Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability. Circulation 132:1909-19 (2015). PubMed: 26416809

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-RAP1 antibody [4c8/1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS-7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    COS-7
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 23 2016

    Western blot abreview for Anti-RAP1 antibody [4c8/1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (GC-1 spg)
    Gel Running Conditions
    Reduced Denaturing (gradient 5-20%)
    Loading amount
    40 µg
    Specification
    GC-1 spg
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 23 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RAP1 antibody [4c8/1] - Telomere Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (nerve)
    Specification
    nerve
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    antibody dilution buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 16 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RAP1 antibody [4c8/1] - Telomere Marker

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 epithelial cell line from mamary gland)
    Specification
    184A1 epithelial cell line from mamary gland
    Fixative
    Methanol and Acetone (1:1)
    Permeabilization
    Yes - Triton X-100 1% in PBS
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    Mr. Peter Zentis

    Verified customer

    投稿 Jul 23 2010

    Question

    I am working on Drosophila Rap1. I need a good antibody to mark it's cellular localization. GTP binding domain in Drosophila and Human is very conserve. May I know which domain did you use to immunize Rap1 antibody? If it is in the conserve regions, this antibody should also work in flies theoretically.

    Read More
    Answer

    The immunogen used to generate this antibody was the Human Rap1 protein. The whole protein was used as an immunogen rather than a peptide sequence. I suggest that you purchase the antibody and determine whether it does cross react with Drosphila and if not, we will refund. Please also let us know if it does cross react by writing a review and in return we'll send you a USD 15.00 Amazon gift voucher.

    Read More

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