Key features and details
- Rabbit polyclonal to RANTES
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
RANTES 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to RANTES
アプリケーション適用あり: WB, Sandwich ELISAmore details
Highly pure (>98%) recombinant hRANTES (human RANTES)
- WB: Recombinant human RANTES protein (ab9680).
Although some customers have been successful in IHC we no longer batch test in this application.
製品の状態Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
バッファーNo preservative, sterile filtered
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab9679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use at an assay dependent concentration.
To detect hRANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
機能Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
組織特異性T-cell and macrophage specific.
配列類似性Belongs to the intercrine beta (chemokine CC) family.
翻訳後修飾N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
- Information by UniProt
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
ab9679 は 18 報の論文で使用されています。
- Zlotkowska A & Andronowska A Variable chemokine expression in porcine trophoblasts and endometrium during the peri-implantation period. Theriogenology 131:16-27 (2019). PubMed: 30928625
- Jin S et al. Activation of cGMP/PKG/p65 signaling associated with PDE5-Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia. Prostate 79:909-919 (2019). PubMed: 30958912
- Xiang P et al. Infiltrating CD4+ T cells attenuate chemotherapy sensitivity in prostate cancer via CCL5 signaling. Prostate 79:1018-1031 (2019). PubMed: 31018021
- Dangaj D et al. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors. Cancer Cell 35:885-900.e10 (2019). PubMed: 31185212
- Sindhu S et al. Increased Adipose Tissue Expression of Interferon Regulatory Factor (IRF)-5 in Obesity: Association with Metabolic Inflammation. Cells 8:N/A (2019). PubMed: 31718015