Anti-RanBP1 抗体 (ab2937)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RanBP1 antibody
RanBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RanBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow
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免疫原
Synthetic peptide corresponding to Human RanBP1 aa 1-18.
Sequence:MAAAKDTHEDHDTSTENT
(Peptide available asab4938) -
ポジティブ･コントロール
- WB: HeLa, Human brain tissue and NIH-3T3 cell lysate. ICC: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2937の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
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| ICC |
Use a concentration of 2 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). |
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ICC
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
配列類似性
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 533251 Cow
- Entrez Gene: 5902 Human
- Entrez Gene: 19385 Mouse
- Omim: 601180 Human
- SwissProt: Q3T0M7 Cow
- SwissProt: P43487 Human
- SwissProt: P34022 Mouse
- Unigene: 24763 Human
see all -
別名
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
画像
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Western blot - Anti-RanBP1 antibody (ab2937)All lanes : Anti-RanBP1 antibody (ab2937) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunocytochemistry - Anti-RanBP1 antibody (ab2937)Immunocytochemistry analysis of HeLa cells labeling RanB1 with ab2937 at 2 µg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated for 3 hours at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing cytoplasmic localization. Panel E shows no primary antibody control. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab2937 は 3 報の論文で使用されています。
- Prpar Mihevc S et al. TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Sci Rep 6:33996 (2016). WB, ICC/IF . PubMed: 27665936
- Štalekar M et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience 293:157-70 (2015). WB, ICC/IF . PubMed: 25743254
- Schulz M et al. Proteomic analysis of peripheral blood mononuclear cells: selective protein processing observed in patients with rheumatoid arthritis. J Proteome Res 6:3752-9 (2007). WB ; Human . PubMed: 17705417
