Anti-RanBP1 抗体 (ab17034)
Key features and details
- Goat polyclonal to RanBP1
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RanBP1 antibody
RanBP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RanBP1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human RanBP1 aa 150 to the C-terminus.
Database link: P43487 -
ポジティブ･コントロール
- WB: Human duodenum lysate. A431, HeLa and MCF7 whole cell lysate. ICC: HEK293 and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).
A 1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 3 - 5 µg/ml.
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| ICC |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
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WB
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa). A 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3 - 5 µg/ml. |
|
ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
配列類似性
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5902 Human
- Omim: 601180 Human
- SwissProt: P43487 Human
- Unigene: 24763 Human
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別名
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
画像
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Western blot - Anti-RanBP1 antibody (ab17034)Anti-RanBP1 antibody (ab17034) at 0.3 µg/ml + Human duodenum lysate (RIPA buffer) at 35 µg
Predicted band size: 23 kDaDetected by chemiluminescence.
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Immunocytochemistry - Anti-RanBP1 antibody (ab17034)Immunofluorescence analysis of paraformaldehyde fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
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Western blot - Anti-RanBP1 antibody (ab17034)Lane 1 : Anti-RanBP1 antibody (ab17034) at 0.03 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab17034) at 0.5 µg/ml
Lane 3 : Anti-RanBP1 antibody (ab17034) at 0.1 µg/ml
Lane 1 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDaDetected by chemiluminescence.
Lysates in RIPA buffer.
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Immunocytochemistry - Anti-RanBP1 antibody (ab17034)Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by Alexa Fluor 488® secondary antibody (2 µg/ml).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab17034 は論文での使用が確認できていません。
