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  1. Link

    rage-antibody-ab3611.pdf

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Anti-RAGE 抗体 (ab3611)

  • Datasheet
  • SDS
Reviews (16)Q&A (16)References (127)

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Western blot - Anti-RAGE antibody (ab3611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
  • Immunohistochemistry (Frozen sections) - Anti-RAGE antibody (ab3611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)

Key features and details

  • Rabbit polyclonal to RAGE
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-RAGE antibody [EPR21171] (ab216329)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-RAGE antibody
    RAGE 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to RAGE
  • 由来種

    Rabbit
  • 特異性

    By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post glycosylation in Mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic Mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.
  • アプリケーション

    適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Rat RAGE aa 362-380.
    Sequence:

    WRKRQPR(R/L)EERKAPESQED


    (Peptide available as ab41778)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: Mouse lung tissue lysate. IHC-P: Mouse lymph node, kidney and heart tissues. IHC-Fr: Transgenic mouse retina.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Sensory System
    • Visual system
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Neuroscience
    • Diseases

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Rat RAGE peptide (ab41778)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human RAGE protein (Fc Chimera Active) (ab219875)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3611の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (1)
Use a concentration of 1 - 2 µg/ml.
WB (6)
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42.6 kDa).Can be blocked with Rat RAGE peptide (ab41778).
IHC-P (1)
1/10 - 1/100.
特記事項
IHC-Fr
Use a concentration of 1 - 2 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42.6 kDa).Can be blocked with Rat RAGE peptide (ab41778).
IHC-P
1/10 - 1/100.

ターゲット情報

  • 機能

    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 組織特異性

    Endothelial cells.
  • 配列類似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q15109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 11596 Mouse
    • SwissProt: Q62151 Mouse
    • Unigene: 3383 Mouse
    • 別名

      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • DAMA 358M23.4 antibody
      • MGC2235 antibody
      • MGC22357 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycation end products antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    画像

    • Western blot - Anti-RAGE antibody (ab3611)
      Western blot - Anti-RAGE antibody (ab3611)

      ab3611 at a 2µg/ml concentration staining ~ 45 kDa RAGE in mouse lung lysate by Western blot (ECL).This antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Frozen sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry (Frozen sections) - Anti-RAGE antibody (ab3611)

      Ab3611 used in IHC (frozen) in transgenic mouse retinas.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab3611)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (127)

    ab3611 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3611 は 127 報の論文で使用されています。

    • Kitaura A  et al. Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages. PLoS One 16:e0245957 (2021). PubMed: 33493233
    • Xue L  et al. Metabolomic profiling identifies a novel mechanism for heat stroke-related acute kidney injury. Mol Med Rep 23:N/A (2021). PubMed: 33655337
    • Crews FT  et al. Loss of Basal Forebrain Cholinergic Neurons Following Adolescent Binge Ethanol Exposure: Recovery With the Cholinesterase Inhibitor Galantamine. Front Behav Neurosci 15:652494 (2021). PubMed: 33716687
    • Qu H  et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
    • Campeão M  et al. Acute MDPV Binge Paradigm on Mice Emotional Behavior and Glial Signature. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 33809599
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 32 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-RAGE antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (fetal membrane)
    Permeabilization
    No
    Specification
    fetal membrane
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 09 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RAGE antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fetal membrane cells)
    Permeabilization
    No
    Specification
    fetal membrane cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 09 2021

    Western blot abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Vascular smooth muscle cell
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 12 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 12 2018

    Western blot abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Breast Cancer Cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Specification
    Human Breast Cancer Cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 13 2018

    Western blot abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse lung epithelial cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    mouse lung epithelial cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 20 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung endothelial cells)
    Permeabilization
    Yes - 0.5% Triton in PBS
    Specification
    Lung endothelial cells
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Acetone:Methanol
    Read More

    Jose Vazquez-Medina

    Verified customer

    投稿 Nov 14 2016

    Western blot abreview for Anti-RAGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole lung tissue lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% BT gel)
    Loading amount
    25 µg
    Specification
    Whole lung tissue lysate
    Blocking step
    Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 25 2016

    Western blot abreview for Anti-RAGE antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (12.5% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (mouse lung)
    Specification
    mouse lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 12 2014

    Question

    Could I use ab3611 for dot blot? Im looking to quantify increased activation pre and post hyperglycemia.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 12 2014

    Answer

    We have not stained dot blots with this antibody but it stains western blots effectively (reduced/denatured samples). I think a western blot would be more convincing data, for example the customer review at this link.

    https://www.abcam.com/RAGE-antibody-ab3611/reviews/3837

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Aug 12 2014

    1-10 of 32 Abreviews or Q&A

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