Anti-Raf1 (phospho S259) 抗体 [EPR3433(2)] (ab173539)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Raf1 (phospho S259) antibody [EPR3433(2)]
Raf1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3433(2)] to Raf1 (phospho S259) -
由来種
Rabbit -
アプリケーション
適用あり: WB, Dot blotmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Treated C6, NIH/3T3 and HEK-293 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3433(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 73 kDa.
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 73 kDa. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
組織特異性
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
関連疾患
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
細胞内局在
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q99N57 Mouse
- SwissProt: P11345 Rat
- Unigene: 159130 Human
see all -
別名
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
画像
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All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaab181602 was used as GAPDH loading control.
ab181115 was used to detect Raf1.
ab150365 was used to detect Raf1 phospho S43.
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All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 5uM forskolin for 10 minutes whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
Lane 1 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution (Purified)
Lanes 2-3 : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) treated with 30uM forskolin for 20 minutes whole cell lysates, then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa -
All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/5000 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour
Lane 3 : HEK293 whole cell lysate treated with 100nM Phorbol-12-myristate-13-acetate for 1 hour then incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Raf1 (pS259) peptide (Lane 1) and Raf1 non-phospho peptide (Lane 2) labelling Raf1 (pS259) with ab173539 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-Raf1 (phospho S259) antibody [EPR3433(2)] (ab173539) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : 293T cell lysates treated with Okadaic Acid + Calyculin A
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab173539 は 13 報の論文で使用されています。
- Li W et al. 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. Cancer Cell Int 22:32 (2022). PubMed: 35042538
- Li J et al. METTL3 Facilitates Tumor Progression by COL12A1/MAPK Signaling Pathway in Esophageal Squamous Cell Carcinoma. J Cancer 13:1972-1984 (2022). PubMed: 35399719
- Bai D et al. BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma. Oncol Lett 24:223 (2022). PubMed: 35720479
- Lin R et al. TRPM2 promotes pancreatic cancer by PKC/MAPK pathway. Cell Death Dis 12:585 (2021). PubMed: 34099637
- Deng D et al. Long non-coding RNA SNHG16 regulates cell behaviors through miR-542-3p/HNF4a axis via RAS/RAF/MEK/ERK signaling pathway in pediatric neuroblastoma cells. Biosci Rep 40:N/A (2020). PubMed: 32412051