Anti-RAD54 抗体 [Rad54 4E3/1] (ab11055)
![Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)](https://www.abcam.co.jp/ps/products/11/ab11055/Images/ab11055-18728-anti-rad54-antibody-rad54-4e3-1-flow-cytometry.jpg "Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)")
Key features and details
- Mouse monoclonal [Rad54 4E3/1] to RAD54
- Suitable for: IP, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-RAD54 antibody [Rad54 4E3/1]
RAD54 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Rad54 4E3/1] to RAD54 -
由来種
Mouse -
アプリケーション
適用あり: IP, WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human RAD54.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Rad54 4E3/1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
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| WB | (2) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8438 Human
- Omim: 603615 Human
- SwissProt: Q92698 Human
- Unigene: 642042 Human
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別名
- DNA repair and recombination protein RAD54 like antibody
- DNA repair and recombination protein RAD54-like antibody
- hHR 54 antibody
see all
画像
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Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab11055 は 6 報の論文で使用されています。
- Zhou Q et al. The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair. Nat Commun 11:2639 (2020). PubMed: 32457312
- Kondrashova O et al. Secondary Somatic Mutations RestoringRAD51CandRAD51DAssociated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma. Cancer Discov 7:984-998 (2017). PubMed: 28588062
- Beck C et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). ICC/IF ; Human . PubMed: 24598253
- Mason JM et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res 74:3546-55 (2014). PubMed: 24753542
- Muylaert I & Elias P Contributions of nucleotide excision repair, DNA polymerase eta, and homologous recombination to replication of UV-irradiated herpes simplex virus type 1. J Biol Chem 285:13761-8 (2010). WB ; Human . PubMed: 20215648
- Murzik U et al. Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11. Mol Biol Cell 19:2926-35 (2008). Human . PubMed: 18463164
