Application
Western blot
Sample
Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount
100 µg
Specification
Embryonic stem cell
Gel Running Conditions
Reduced Denaturing (8% page gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
1 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xpbs, 0.2% tween 20, 3% milk
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immuno Goat Anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: ~37 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 27 2012