Anti-Rad51 抗体 (ab63801)
Key features and details
- Rabbit polyclonal to Rad51
- Suitable for: WB, ICC/IF
- Reacts with: Human, Xenopus laevis
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Rad51 antibody
Rad51 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rad51 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Xenopus laevis -
免疫原
Purified recombinant full length protein (Human)
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ポジティブ・コントロール
- WB: Crude extract of Xenopus; HeLa cell extract. ICC: U2OS cells.
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特記事項
Please click here for Instructions For Use/Protocol.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.5
Preservative: 0.09% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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ICC/IF | (5) |
1/100.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
1/100. |
ターゲット情報
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機能
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
組織特異性
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
関連疾患
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
配列類似性
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
ドメイン
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
翻訳後修飾
Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
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別名
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
画像
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2BN hTert (XLF-deficient) human fibroblasts were fixed for 30 min with methanol at −20°C, dipped for 1 min in ice cold acetone for permeabilization and washed three times for 10 min with PBS/1% FCS. Non-specific antigens were blocked for 30 min in 5% BSA in PBS/1% FCS. Samples were incubated with ab63801 (1∶15000) in PBS/1% FCS over night at 4°C, washed three times in PBS/1% FCS and incubated for 1 h at room temperature with AlexaFluor 488 conjugated secondary antibodies (1 : 500). After three times of washing in PBS, cells were DAPI stained.
Image shows 2BN hTert (XLF-deficient) human fibroblasts analyzed 2 h post IR with 1 Gy.
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Anti-Rad51 antibody (ab63801) at 1/1000 dilution + Crude extract of Xenopus
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence analysis of human osteosarcoma U2OS cells X-ray irradiation before and 2h after, immunostained with Anti-Rad51 antibody (ab63801) at 1/6,000 dilution. The lower panels were the same cells with Hoechst.
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All lanes : Anti-Rad51 antibody (ab63801) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : HeLa cell extract
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801 (x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (114)
ab63801 は 114 報の論文で使用されています。
- Hariharasudhan G et al. TOPORS-mediated RAD51 SUMOylation facilitates homologous recombination repair. Nucleic Acids Res 50:1501-1516 (2022). PubMed: 35061896
- Shih DJH et al. Exploiting induced vulnerability to overcome PARPi resistance and clonal heterogeneity in BRCA mutant triple-negative inflammatory breast cancer. Am J Cancer Res 12:337-354 (2022). PubMed: 35141022
- Kolinjivadi AM et al. The H. pylori CagA Oncoprotein Induces DNA Double Strand Breaks through Fanconi Anemia Pathway Downregulation and Replication Fork Collapse. Int J Mol Sci 23:N/A (2022). PubMed: 35163588
- Xin Y et al. Identification of Nanog as a novel inhibitor of Rad51. Cell Death Dis 13:193 (2022). PubMed: 35220392
- Ma H et al. HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer. Cell Death Dis 13:263 (2022). PubMed: 35332131