Application
ChIP
Sample
Human Cell lysate - nuclear (293T)
Negative control
IgG control
Specification
293T
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA
Positive control
GAPDH gene
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Chip binding buffer
Dilution
1/500
Additional data
Additional Notes
Cells UV treated before performing CHIP
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 02 2019