Anti-Rad51 抗体 [14B4] (ab213)
Key features and details
- Mouse monoclonal [14B4] to Rad51
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Rad51 antibody [14B4]
Rad51 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [14B4] to Rad51 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Rad51 aa 1-338.
Database link: Q06609 -
ポジティブ・コントロール
- WB: RAD51 shRNA transfected (+) 293T whole cell extracts, Jurkat, Raji and NCI-H929 whole cell lysate. IHC-P: BT483 xenoograft tissue, human colon esophagus tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
14B4 -
ミエローマ
NS1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100 - 1/1000.
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WB | (5) |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
Please see WB protocol in image legend. |
特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. Please see WB protocol in image legend. |
ターゲット情報
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機能
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
組織特異性
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
関連疾患
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
配列類似性
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
ドメイン
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
翻訳後修飾
Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
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別名
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
画像
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All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa10% SDS-PAGE
Running condition: 80V, 15min; 140V, 40 min
Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60 min.
Primary antibody incubation: 4°C overnight.
Washing condition: 5 ml TBST, 4 x 5 min.
Chemiluminescent HRP Substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
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All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1 : Non-transfected 293T (-) whole cell extracts
Lane 2 : RAD51 shRNA transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 37 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.
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Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg
Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 42,45 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)This image is courtesy of an anonymous Abreview
ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (74)
ab213 は 74 報の論文で使用されています。
- Lin M et al. LINC00200 contributes to the chemoresistance to oxaliplatin of gastric cancer cells via regulating E2F1/RAD51 axis. Hum Cell 34:1163-1173 (2021). PubMed: 33822325
- Salmina K et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci 21:N/A (2020). PubMed: 32316332
- Shan W et al. Systematic Characterization of Recurrent Genomic Alterations in Cyclin-Dependent Kinases Reveals Potential Therapeutic Strategies for Cancer Treatment. Cell Rep 32:107884 (2020). PubMed: 32668240
- Sinha A et al. RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational Status. Cancers (Basel) 12:N/A (2020). PubMed: 33138032
- Horikoshi N et al. Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase. Commun Biol 2:253 (2019). PubMed: 31286070