製品の概要
-
製品名Anti-Rad51 antibody [14B4]
Rad51 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [14B4] to Rad51
-
由来種Mouse
-
特異性Specific for human Rad51.
-
アプリケーション適用あり: IHC-P, WB, IPmore details
適用なし: ICC/IF -
種交差性交差種: Human
-
免疫原
Recombinant full length protein corresponding to Human Rad51 aa 1-338.
-
ポジティブ･コントロール
- T24 Bladder carcinoma cells and recombinant fusion protein.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
バッファーConstituents: PBS, pH 7.4
-
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノモノクローナル
-
クローン名14B4
-
ミエローマNS1
-
アイソタイプIgG2b
-
軽鎖の種類kappa
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/200. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. | |
| IP | Use at an assay dependent concentration. |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
-
機能Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
-
組織特異性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
-
関連疾患Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
配列類似性Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
ドメインThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
-
翻訳後修飾Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
細胞内局在Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
- Information by UniProt
-
参照データベース
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
-
別名
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
画像
-
![Western blot - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.co.jp/ps/products/0/ab213/Images/ab213-275402-ab213WB.jpg)
Western blot - Anti-Rad51 antibody [14B4] (ab213)Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + Jurkat whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 37 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.co.jp/ps/products/0/ab213/Images/ab213-216007-ab213ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of BT483 xenograft labeling RAD51 with ab213 at 1/200.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.co.jp/ps/products/0/ab213/Images/ab213-200211-ab213ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)This image is courtesy of an anonymous Abreviewab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
-
![Western blot - Anti-Rad51 antibody [14B4] (ab213)](https://www.abcam.co.jp/ps/products/0/ab213/Images/Rad51-Primary-antibodies-ab213-6.jpg)
Western blot - Anti-Rad51 antibody [14B4] (ab213)Image courtesy of an anonymous Abreview.Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg
Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 42,45 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
参考文献
This product has been referenced in:
- Roy S et al. p53 orchestrates DNA replication restart homeostasis by suppressing mutagenic RAD52 and POL? pathways. Elife 7:N/A (2018). Read more (PubMed: 29334356) »
- Bleuyard JY et al. MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress. Proc Natl Acad Sci U S A 114:7671-7676 (2017). WB . Read more (PubMed: 28673974) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"