Application
Western blot
Sample
Mouse Cell lysate - whole cell (293T and MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T and MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Cell Signalling Anti-mouse IgG, HRP-linked Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140 kDa Non-specific: none kDa
Positive control
293T
Additional data
Additional Notes
Great antibody, though very weak in mouse fibroblasts.
Abcam response
We would like to thank the reviewer for submitting this AbReview for this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 22 2018