Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Primary hepatoctyes)
Specification
Primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: I-Block buffer
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/15000
Detection
Detection method
ECL
Bands
Specific: 130 kDa Non-specific: 40, 42, 57, 70, 80, 100 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
ok
Additional data
Additional Notes
Why we can not detect the 72KDa band?
Abcam response
Thank you for your feedback. Reviewing other customer feedback, I believe the correct band is at ~130kDa. Rad21 does not seem to run at the predicted size and a paper has been published that documents this in more detail:
Mol Cell Biol. 2002 Dec;22(23):8267-77 PMID: 12417729.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Shengyun Ma
Verified customer
投稿 Aug 09 2013