Anti-Rad21 抗体 (ab992)

Reviews (19)Q&A (9)References (137)
Western blot - Anti-Rad21 antibody (ab992)
  • Immunoprecipitation - Anti-Rad21 antibody (ab992)

Key features and details

  • Rabbit polyclonal to Rad21
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Rad21 antibody
    Rad21 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Rad21

  • 由来種

    Rabbit

  • 特異性

    The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant

  • 免疫原

    Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).

  • ポジティブ･コントロール

    • WB: HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH/3T3, and TCMK-1 whole cell lysates.

  • 特記事項

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab992の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP (1)
Use at an assay dependent concentration.

ab992 used for IP at 6 µg per reaction.
WB (7)
1/500 - 1/5000. Predicted molecular weight: 72 kDa.

Band observed at ~130 kDa.
特記事項
IP
Use at an assay dependent concentration.

ab992 used for IP at 6 µg per reaction.
WB
1/500 - 1/5000. Predicted molecular weight: 72 kDa.

Band observed at ~130 kDa.

ターゲット情報

  • 機能

    Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

  • 配列類似性

    Belongs to the rad21 family.

  • ドメイン

    The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.

  • 翻訳後修飾

    Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
    Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.

  • 細胞内局在

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CDLS4 antibody
    • Double-strand-break repair protein rad21 homolog antibody
    • hHR21 antibody
    • HR21 antibody
    • HRAD21 antibody
    • KIAA0078 antibody
    • MCD1 antibody
    • Nuclear matrix protein 1 antibody
    • NXP-1 antibody
    • NXP1 antibody
    • Protein involved in DNA double-strand break repair antibody
    • RAD21 antibody
    • RAD21 homolog (S. pombe) antibody
    • RAD21 homolog antibody
    • RAD21_HUMAN antibody
    • Scc1 antibody
    • SCC1 homolog antibody
    see all

画像

  • Western blot - Anti-Rad21 antibody (ab992)
    Western blot - Anti-Rad21 antibody (ab992)
    All lanes : Anti-Rad21 antibody (ab992) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : RKO (human cell line from colon carcinoma) whole cell lysate
    Lane 5 : GaMG (human cell line from glioblastoma) whole cell lysate
    Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 8 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 9 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 10 : TCMK-1 (mouse epithelial kidney cell line transformed with SV40) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-Rad21 antibody (ab992)
    Immunoprecipitation - Anti-Rad21 antibody (ab992)

    Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer.

    Lane 1: ab992 used for IP at 6 µg per reaction

    Lane 2: Control IgG

    For blotting immunoprecipitated Rad21, a rabbit anti-Rad21 recombinant monoclonal antibody was used at 1/1000. Detected using chemiluminescence with an exposure time of 3 minutes.

参考文献 (137)

ab992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab992 は 137 報の論文で使用されています。

  • Kentepozidou E  et al. Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains. Genome Biol 21:5 (2020). PubMed: 31910870
  • Khoury A  et al. Constitutively bound CTCF sites maintain 3D chromatin architecture and long-range epigenetically regulated domains. Nat Commun 11:54 (2020). PubMed: 31911579
  • Jia Z  et al. Tandem CTCF sites function as insulators to balance spatial chromatin contacts and topological enhancer-promoter selection. Genome Biol 21:75 (2020). PubMed: 32293525
  • McNicoll F  et al. Meiotic sex chromosome cohesion and autosomal synapsis are supported by Esco2. Life Sci Alliance 3:N/A (2020). PubMed: 32051254
  • Nishana M  et al. Defining the relative and combined contribution of CTCF and CTCFL to genomic regulation. Genome Biol 21:108 (2020). PubMed: 32393311
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 28 Abreviews or Q&A

Question

Customer kindly contacted us regarding positve and negative primers for our ChIP grade antibodies.

Read More
Answer

I've compiled some information below regarding potential primer sets for the ChIP antibodies we talked about, where information was lacking (verified in ChIP by other researches), I included links to abReviews and references that used the antibody in ChIP.

1. ab817: GAPDH (+), GAD1 (-), AFM (-), Factor VIII (-)

2. ab1791: GAPDH, RPL30, ALDOA, MYO-D, SERPINA, AFM, SAT2, SATa (each batch is verified against this primer set, an example image of one batch test is given on the datasheet)

3. ab7030: was verified in ChIP by other researchers submitting data through our abReview system and specific references. Their positive and negative controls used are given through the link below, as well as a "click to contact this user" link that allows you to contact the researchers if you would like more specific primer information. The specific references with ChIP listed in bold are good sources for positive and negative primers as well.
a) Abreview: (NFKBIA promoter (+), GAPDH (-)) https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html?intabreviewid=25057
b) Abreview: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html?intabreviewid=18386
c) Specific references: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-references.html

4. ab111069 Untested in ChIP.

5. ab992: was verified in ChIP by other researchers (abReviews and specific references)
a) Specific references: https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-references.html
b) Abreview: https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-reviews.html?intabreviewid=14542
c) Abreview: (EBV 3+ (+), EBV1,2,4,5,6 (-), GAPDH (-), Cen (-)) https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-reviews.html?intabreviewid=33942

Our ChIP primer sets:
https://www.abcam.com/index.html?pageconfig=resource&rid=10600

Read More

ChIP abreview for Anti-Rad21 antibody - ChIP Grade

 Good
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (MCF10a)
Negative control
Random regions without ATAC-seq signal or any histone marks.
Specification
MCF10a
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control
Strong CTCF binding sites
Read More

Abcam user community

Verified customer

投稿 Dec 10 2019

ChIP abreview for Anti-Rad21 antibody - ChIP Grade

 Below Average
Abreviews
abreview image
Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Limb buds, forebrain, ESC)
Negative control
We perform ChIP-seq
Specification
Limb buds, forebrain, ESC
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% Formaldehyde (Sigma), dilu
Positive control
We perform ChIP-seq
Read More

Daniel Ibrahim

Verified customer

投稿 Nov 12 2018

Western blot abreview for Anti-Rad21 antibody - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Whole cell lysate resolved on a 12% SDS PAGE gel)
Gel Running Conditions
Reduced Denaturing (12% SDS gel)
Loading amount
25 µg
Treatment
HEK293T cells transfected with HA-Rad21 plasmid for overexpression
Specification
Whole cell lysate resolved on a 12% SDS PAGE gel
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 19 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad21 antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Cells are incubated with primary antibody + BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Sample
Donkey Cell (Skin fibroblasts)
Specification
Skin fibroblasts
Permeabilization
Yes - 1% PFA + 0.2% Triton
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 12 2014

Question

What does this antibody recognize on human Rad21?

Read More
Answer

The epitope recognized by ab992 maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad21 antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (primary hepatoctyes)
Specification
primary hepatoctyes
Permeabilization
Yes - Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Fixative
Paraformaldehyde
Read More

Mr. Shengyun Ma

Verified customer

投稿 Oct 07 2013

Immunoprecipitation abreview for Anti-Rad21 antibody - ChIP Grade

 Below Average
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - whole cell (Hep3B)
Specification
Hep3B
Total protein in input
30 µg
Read More

Abcam user community

Verified customer

投稿 Sep 16 2013

ChIP abreview for Anti-Rad21 antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
Suz12 on h19 ICR
Read More

Abcam user community

Verified customer

投稿 Sep 16 2013

Western blot abreview for Anti-Rad21 antibody - ChIP Grade

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Primary hepatoctyes)
Specification
Primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 4°C
Read More

Mr. Shengyun Ma

Verified customer

投稿 Aug 09 2013

1-10 of 28 Abreviews or Q&A

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