Anti-RACO-1 抗体 (ab272604)
Key features and details
- Rabbit polyclonal to RACO-1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RACO-1 antibody -
製品の詳細
Rabbit polyclonal to RACO-1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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免疫原
Recombinant fragment corresponding to Human RACO-1 aa 150-250.
Database link: Q5TA31 -
ポジティブ･コントロール
- IHC-P: Human testis tissue. ICC/IF: U-2 OS.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 RING-type zinc finger. -
ドメイン
The RING-type zinc finger domain is required for E3 ligase activity. -
翻訳後修飾
Ubiquitinated; undergoes 'Lys-48'-linked autoubiquitination in the absence of growth factors and MAP3K1-induced 'Lys-63'-linked polyubiquitination. 'Lys-48'-autoubiquitination leads to degradation by the proteasome, while MAP3K1-induced 'Lys-63'-linked polyubiquitination results in the stabilization of the protein. 'Lys-48'- and 'Lys-63'-linked polyubiquitinations occur most probably on the same 3 C-terminal lysine residues (Lys-195, Lys-223 and Lys-224) and are thus mutually exclusive. Other sites of ubiquitination are not excluded.
Arginine methylation by PRMT1 stabilizes RNF187 by facilitating K63-linked ubiquitin chain formation, and enables dimerization, c-Jun interaction and subsequent AP1 target gene expression. -
細胞内局在
Cytoplasm. Nucleus. Predominantly located in the cytoplasm. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 149603 Human
- Entrez Gene: 108660 Mouse
- Entrez Gene: 360533 Rat
- Omim: 613754 Human
- SwissProt: Q5TA31 Human
- SwissProt: Q8BFX1 Mouse
- SwissProt: D3Z8N2 Rat
- Unigene: 356377 Human
see all -
別名
- E3 ubiquitin-protein ligase RNF187 antibody
- Protein RNF187 antibody
- RACO-1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACO-1 antibody (ab272604)
Paraffin-embedded formalin-fixed Human testis tissue stained for RACO-1 using ab272604 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-RACO-1 antibody (ab272604)PFA fixed, Triton X-100 permeablized human cell line U-2 labeling RACO-1 (green) using ab272604 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272604 は論文での使用が確認できていません。
