Anti-RAC3 抗体 [EPR6679(B)] - BSA and Azide free (ab248066)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6679(B)] to RAC3 - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAC3 antibody [EPR6679(B)] - BSA and Azide free
RAC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6679(B)] to RAC3 - BSA and Azide free -
由来種
Rabbit -
特異性
This antibody cross-reacts with Rac1, Rac2 and Cdc42.
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アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248066 is the carrier-free version of ab124943.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.23 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6679(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248066の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. -
組織特異性
Highest levels in brain, also detected in heart, placenta and pancreas. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
翻訳後修飾
(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly. -
細胞内局在
Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery. - Information by UniProt
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参照データベース
- Entrez Gene: 5881 Human
- Entrez Gene: 170758 Mouse
- Omim: 602050 Human
- SwissProt: P60763 Human
- SwissProt: P60764 Mouse
- Unigene: 45002 Human
- Unigene: 34008 Mouse
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製品の状態
Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
別名
- OTTMUSP00000004488 antibody
- p21 Rac3 antibody
- p21-Rac3 antibody
see all
画像
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All lanes : Anti-RAC3 antibody [EPR6679(B)] (ab124943) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : JAR cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDaThis data was developed using ab124943, the same antibody clone in a different buffer formulation.
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This data was developed using ab124943, the same antibody clone in a different buffer formulation.ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human heart tissue by immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab124943, the same antibody clone in a different buffer formulation.ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human placenta tissue by immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab124943, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248066 は論文での使用が確認できていません。