Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6679(B)] to RAC3 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-RAC3 antibody [EPR6679(B)] - BSA and Azide free
RAC3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6679(B)] to RAC3 - BSA and Azide free
This antibody cross-reacts with Rac1, Rac2 and Cdc42.
アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
適用なし: Flow Cyt or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human RAC3 aa 50-150. The exact sequence is proprietary.
Ab248066 is the carrier-free version of ab124943. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248066 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 1.23 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
機能Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.
組織特異性Highest levels in brain, also detected in heart, placenta and pancreas.
配列類似性Belongs to the small GTPase superfamily. Rho family.
翻訳後修飾(Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
細胞内局在Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.
- Information by UniProt
製品の状態Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
- OTTMUSP00000004488 antibody
- p21 Rac3 antibody
- p21-Rac3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.