Anti-RAC2 抗体 (ab2244)
Key features and details
- Goat polyclonal to RAC2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RAC2 antibody
RAC2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RAC2 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
PQPTRQQKRACSLL
, corresponding to C terminal amino acids 179-192 of Human RAC2. -
ポジティブ･コントロール
- Recombinant Human RAC2 protein (ab78684) can be used as a positive control in WB. MOLT4 or liver lysate.
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特記事項
Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa. A 1 hour primary incubation is recommended for this product. |
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| IHC-P | Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative. |
ターゲット情報
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機能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
組織特異性
Hematopoietic specific. -
関連疾患
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
細胞内局在
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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参照データベース
- Entrez Gene: 5880 Human
- Entrez Gene: 19354 Mouse
- Omim: 602049 Human
- SwissProt: P15153 Human
- SwissProt: Q05144 Mouse
- Unigene: 517601 Human
- Unigene: 1972 Mouse
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製品の状態
Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
別名
- EN 7 antibody
- EN-7 antibody
- EN7 antibody
see all
画像
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Western blot - Anti-RAC2 antibody (ab2244)All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
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Western blot - Anti-RAC2 antibody (ab2244)Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
参考文献 (8)
ab2244 は 8 報の論文で使用されています。
- Xia P et al. Down-regulation of RAC2 by small interfering RNA restrains the progression of osteosarcoma by suppressing the Wnt signaling pathway. Int J Biol Macromol 137:1221-1231 (2019). PubMed: 31279058
- Gerasimcik N et al. The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice. Front Immunol 8:1264 (2017). PubMed: 29056938
- Assani K et al. Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia. J Immunol 198:1985-1994 (2017). PubMed: 28093527
- Sowrirajan B et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
- Hinterseher I et al. Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci 16:11196-212 (2015). PubMed: 25993291
- Kawazu M et al. Transforming mutations of RAC guanosine triphosphatases in human cancers. Proc Natl Acad Sci U S A 110:3029-34 (2013). Human . PubMed: 23382236
- Tomilov AA et al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB ; Mouse . PubMed: 19892704
- Ramgolam VS et al. T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade. PLoS One 5:e14450 (2010). WB ; Human . PubMed: 21206905
