Anti-RAC1 + Cdc42 (phospho S71) 抗体 (ab5482)

Reviews (1)Q&A (2)References (6)

Key features and details

  • Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-RAC1 + Cdc42 (phospho S71) antibody
    RAC1 + Cdc42 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)

  • 由来種

    Rabbit

  • 特異性

    Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic phospho peptide (Human) containing serine 71. The sequence is conserved in human and mouse RAC 1, 2, and 3, and Cdc 42 human, mouse, rat, dog and frog.

  • ポジティブ･コントロール

    • A431 cells treated with EGF.

  • 特記事項


    RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RAC 1. The final product is generated by affinity chromatography using a RAC 1 derived peptide that is phosphorylated at serine 71.

  • 一次抗体 備考

    RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 23 kDa.

ターゲット情報

  • 関連性

    Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.

  • 細胞内局在

    Cell membrane; Lipid anchor; Cytoplasmic side.

  • 参照データベース

    • 別名

      • CDC42Hs antibody
      • Cell division cycle 42 antibody
      • G25K antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • p21 Rac1 antibody
      • Ras like protein TC25 antibody
      • Small GTP binding protein Cdc42 antibody
      • TC25 antibody
      see all

    画像

    • Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
      Peptide Competition and Phosphatase Treatment:
      Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5482 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the peptide corresponding to ab5482 blocks the antibody signal. The data also shows that phosphatase stripping eliminates the signal, verifying that the antibody is pho

    参考文献 (6)

    ab5482 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5482 は 6 報の論文で使用されています。

    • Li N  et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). PubMed: 30837867
    • Kang DS  et al. Netrin-1/DCC-mediated PLC?1 activation is required for axon guidance and brain structure development. EMBO Rep 19:N/A (2018). PubMed: 30224412
    • Maurya VK  et al. Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse. J Ovarian Res 7:32 (2014). PubMed: 24628852
    • Haas S  et al. DIGE proteome analysis reveals suitability of ischemic cardiac in vitro model for studying cellular response to acute ischemia and regeneration. PLoS One 7:e31669 (2012). WB ; Mouse . PubMed: 22384053
    • Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB ; Human . PubMed: 18978815
    • Tan AM  et al. Neuropathic pain memory is maintained by Rac1-regulated dendritic spine remodeling after spinal cord injury. J Neurosci 28:13173-83 (2008). WB ; Rat . PubMed: 19052208

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    Western blot abreview for Anti-RAC1 + Cdc42 (phospho S71) antibody

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    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Aug 16 2017

    Question

    What is the IgG concentration? The lot number is GR67743.

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    Answer

    The antibody concentration of the lot you have is 0.35 mg/ml.

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    Question

    I have a question regarding your Rac/Cdc42 antibody: As mentioned in the description for your RAC antibody [23A8], rac shows a band at 21 kDa, while the CDC42 antibody (ab14241)shows a band at 24 kDa. Does the Rac/Cdc42 antibody (a5482) that recognizes both proteins display two seperate bands which would allow to quantify the the amount of Cdc42 and Rac in one blot or is it just one broad band? Thanks for your help

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    Answer

    This antibody recognizes a sequence that is exactly conserved amongst the proteins. We would thus expect that providing you resolve your gel in order to separate the two proteins, the antibody should recognise the two proteins separately.

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