Anti-Rac1 抗体 (ab97732)
Key features and details
- Rabbit polyclonal to Rac1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rac1 antibody
Rac1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rac1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic non phosphopeptide derived from around amino acids 69-73 of Human Rac1 (
PLSYP
). -
ポジティブ・コントロール
- Recombinant Human Rac1 protein (ab51014) can be used as a positive control in WB. HeLa cells; A431 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab97732 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97732の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. -
組織特異性
Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
ドメイン
The effector region mediates interaction with DEF6. -
翻訳後修飾
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
細胞内局在
Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 5879 Human
- Omim: 602048 Human
- SwissProt: P63000 Human
- Unigene: 413812 Human
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製品の状態
Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity. -
別名
- Cell migration inducing gene 5 protein antibody
- Cell migration-inducing gene 5 protein antibody
- MGC111543 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab97732 は 9 報の論文で使用されています。
- Yang TY et al. Mulberry Leaf and Neochlorogenic Acid Alleviates Glucolipotoxicity-Induced Oxidative Stress and Inhibits Proliferation/Migration via Downregulating Ras and FAK Signaling Pathway in Vascular Smooth Muscle Cell. Nutrients 14:N/A (2022). PubMed: 35893859
- Liu L et al. Development of a Toll-Like Receptor-Based Gene Signature That Can Predict Prognosis, Tumor Microenvironment, and Chemotherapy Response for Hepatocellular Carcinoma. Front Mol Biosci 8:729789 (2021). PubMed: 34621787
- Chen D et al. 6-Thioguanine Inhibits Herpes Simplex Virus 1 Infection of Eyes. Microbiol Spectr 9:e0064621 (2021). PubMed: 34730435
- Xia PP et al. Rac1 relieves neuronal injury induced by oxygenglucose deprivation and re-oxygenation via regulation of mitochondrial biogenesis and function. Neural Regen Res 15:1937-1946 (2020). PubMed: 32246643
- Zhang X et al. MicroRNA 483-3p targets Pard3 to potentiate TGF-ß1-induced cell migration, invasion, and epithelial-mesenchymal transition in anaplastic thyroid cancer cells. Oncogene 38:699-715 (2019). PubMed: 30171257
- Li G et al. Acupuncture Administration Improves Cognitive Functions and Alleviates Inflammation and Nuclear Damage by Regulating Phosphatidylinositol 3 Kinase (PI3K)/Phosphoinositol-Dependent Kinase 1 (PDK1)/Novel Protein Kinase C (nPKC)/Rac 1 Signaling Pathway in Senescence-Accelerated Prone 8 (SAM-P8) Mice. Med Sci Monit 25:4082-4093 (2019). PubMed: 31152645
- Liu B et al. Knockdown of lncRNA LSINCT5 suppresses growth and metastasis of human glioma cells via up-regulating miR-451. Artif Cells Nanomed Biotechnol 47:2507-2515 (2019). PubMed: 31213092
- Feng H et al. Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation. Nanoscale Res Lett 11:240 (2016). ICC/IF ; Rat . PubMed: 27142878
- Montagner A et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF ; Mouse . PubMed: 24203162