Anti-Rac1 + Rac2 + Rac3 抗体 [EPR18631] (ab180683)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631]
Rac1 + Rac2 + Rac3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant human Rac1, Rac2 and Rac3 full length protein. Human fetal brain lysate. HEK-293, HeLa, HUVEC, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18631 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180683の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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特記事項 |
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WB
1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
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関連性
Rac1 function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. GTP hydrolysis is stimulated by ARHGAP30. Rac2 fucntion: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Rac3 function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner. -
参照データベース
- Entrez Gene: 5879 Human
- Entrez Gene: 5880 Human
- Entrez Gene: 5881 Human
- Entrez Gene: 170758 Mouse
- Entrez Gene: 19353 Mouse
- Entrez Gene: 19354 Mouse
- Entrez Gene: 363875 Rat
- Entrez Gene: 366957 Rat
see all -
別名
- Cell migration-inducing gene 5 protein antibody
- EN-7 antibody
- GX antibody
see all
画像
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All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : Recombinant Human Rac1 full length protein
Lane 2 : Recombinant Human Rac2 full length protein
Lane 3 : Recombinant Human Rac3 full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:
Lanes 1 & 2:1 second.
Lane 3: 3 minutes.
Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac’s were made in house.
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All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2-4 : 15 seconds.
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All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab180683 は 4 報の論文で使用されています。
- Chen L et al. MTSS1 inhibits colorectal cancer metastasis by regulating the CXCR4/CXCL12 signaling axis. Int J Mol Med 47:N/A (2021). PubMed: 33649808
- Mei L et al. The Novel Antioxidant Compound JSH-23 Prevents Osteolysis by Scavenging ROS During Both Osteoclastogenesis and Osteoblastogenesis. Front Pharmacol 12:734774 (2021). PubMed: 34566656
- Chen Y et al. Catalpol Ameliorates Podocyte Injury by Stabilizing Cytoskeleton and Enhancing Autophagy in Diabetic Nephropathy. Front Pharmacol 10:1477 (2019). PubMed: 31920663
- Hu J et al. Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway. Oncol Lett 16:2312-2318 (2018). PubMed: 30008934