Anti-RAC1 + Cdc42 (phospho S71) 抗体 [EP221Y] (ab76535)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y]
RAC1 + Cdc42 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A431 cells treated with Lambda Phosphatase.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP221Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76535の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 23 kDa.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 23 kDa. |
ターゲット情報
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関連性
Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK. -
細胞内局在
Cell membrane; Lipid anchor; Cytoplasmic side. -
参照データベース
- Entrez Gene: 5879 Human
- Entrez Gene: 998 Human
- Omim: 116952 Human
- Omim: 602048 Human
- SwissProt: P60953 Human
- SwissProt: P63000 Human
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別名
- CDC42Hs antibody
- Cell division cycle 42 antibody
- G25K antibody
see all
画像
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All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution
Lane 1 : A431 cell lysate treated with Lambda Phosphatase
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 24 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab76535 は 1 報の論文で使用されています。
- Liu Y et al. Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes Dis 8:232-240 (2021). PubMed: 33997170