Rabbit Anti-Syrian Hamster IgG H&L (HRP) (ab6783)

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Key features and details

  • Rabbit Anti-Syrian Hamster IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ICC, Dot blot, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Syrian Hamster IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Syrian hamster

  • アプリケーション

    適用あり: ICC, Dot blot, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, ELISAmore details

  • 免疫原

    hamster IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Lyophilized:Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    IgG fraction

  • 特記事項（精製）

    Horseradish Peroxidase (HRP).

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ICC/IF 1/500 - 1/2500.
ELISA 1/16000 - 1/64000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab6783 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6783 は 1 報の論文で使用されています。

  • Kogianni G  et al. Fas/CD95 is associated with glucocorticoid-induced osteocyte apoptosis. Life Sci 75:2879-95 (2004). PubMed: 15454340

レビューと Q&A

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Question

Are your secondary antibodies made against hamster IgG made against Armenian hamster IgG? (e.g. #66783, 6892, 7146)? Approximately what dilution of them would you recommend for an ELISA assay? Are there any pros or cons to rabbit vs. goat? Thanks, K. Spindler

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Answer

These antibodies are made against Golden Syrian hamster. The ELISA data suggest the following dilutions: ab6783 is 1:4000 - 1:20,000, ab6892 is 1:15,000 -1:65,000,and ab7146 is 1:4,000 - 1:20,000. There should be no difference in the hosts - it simply provides a choice. However, goat host has a minimal cross-reactivity and rabbit has anti-F(ab')2. We will soon have antisera and IgG fractions to Armenian hamster and are working on affinity purified antisera, but that will take some months.

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