Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)

Submit a review Q&A (1)References (4)

Key features and details

  • Rabbit Anti-Sheep IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Sheep IgG H&L (Texas Red ®)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • 免疫原

    Sheep IgG whole molecule

  • 標識

    Texas Red ®. Ex: 596nm, Em: 620nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • 特記事項

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. PubMed: 17895403
ELISA 1/10000 - 1/50000.

画像

  • Immunocytochemistry - Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)
    Immunocytochemistry - Rabbit Anti-Sheep IgG H&L (Texas Red ®) (ab6745)
    Ab6745 was used at dilution 1/100 with the primary antibody ab30524 in ICC/IF. See the review on ab30524.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab6745 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6745 は 4 報の論文で使用されています。

  • Kirby BS  et al. Robust internal elastic lamina fenestration in skeletal muscle arteries. PLoS One 8:e54849 (2013). IHC . PubMed: 23359815
  • Pawlak E  et al. Distribution and processing of a disintegrin and metalloproteinase with thrombospondin motifs-4, aggrecan, versican, and hyaluronan in equine digital laminae. Am J Vet Res 73:1035-46 (2012). PubMed: 22738056
  • Jones S  et al. Peripheral tachykinins and the neurokinin receptor NK1 are required for platelet thrombus formation. Blood 111:605-12 (2008). ICC/IF . PubMed: 17895403
  • Czaja K  et al. Leptin receptors, NPY, and tyrosine hydroxylase in autonomic neurons supplying fat depots in a pig. Biochem Biophys Res Commun 293:1138-44 (2002). PubMed: 12051778

レビューと Q&A

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Question

Hello I am working on a project to identify human cells in sheep tissues, specifically cardiac muscle, skeletal muscle, and liver. We are interested in your product ab259, the sheep polyclonal ab to human transferrin. What kind of cross reactivity does this antibody have to sheep? What are typical dilutions when using the ab for immunocytochemistry? What concentration does the ab come in? Finally, can you recommend another ab for identifying human cells in sheep? Thanks for your time! Evan Colletti

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Answer

This antibody has not been tested in sheep but is unlikely to cross-react as this polyclonal was raised in this species. The IgG concentration, however, should be in the region of 18.6mg/ml - as this is an IgG fraction, specific IgG will be approximately 10% of this.

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