Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed (ab7111)

Submit a review Q&A (4)References (3)

Key features and details

  • Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr

製品の概要

  • 製品名

    Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Full length native Sheep IgG (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Horseradish Peroxidase (HRP)

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300 - 1/1500.
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/3000.
ELISA 1/5000 - 1/22000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab7111 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7111 は 3 報の論文で使用されています。

  • Dinamarca MC  et al. Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing. Nat Commun 10:1331 (2019). PubMed: 30902970
  • Schmidt N  et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
  • Kristiansson MH  et al. Comparative time-dependent analysis of potential inflammation biomarkers in lymphoma-bearing SJL mice. J Proteome Res 6:1735-44 (2007). PubMed: 17388619

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

I received the Ab but unfortunately it doesn’t work!! I had tried the previous, contaminated, batch just in case it worked but it didn’t. We do have a positive control which we tested (an older Abcam anti sheep-HRP) which we got an aliquot of from a colleague and this worked. But on the same protein extracts and same primary Ab, testing of the two batches none have worked.

I see that you shipped batch GR5200-6. When I phoned I am certain I was told that that batch was also contaminated. Could you please check?

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Answer

Thank you for your answer.

We had checked the lot GR5200-6 and have not seen a contamination, this is why we have sent you one vial of this lot.

I am very sorry however to hear that the new vial does not work for you. We will be pleased to send you an alternative antibody as a replacement as we do not have any other lots of this antibody in stock. Can you please confirm in what dilution you have tested this antibody and it does not work?

As alternative antibodies, I can recommend the ab97130 or the ab6747 for example.https://www.abcam.com/index.html?datasheet=97130 (or use the following: https://www.abcam.com/index.html?datasheet=97130). https://www.abcam.com/index.html?datasheet=6747 (or use the following: https://www.abcam.com/index.html?datasheet=6747).

Please do let me know whether you would like to accept an alternative antibody or if you prefer to receive a credit note or refund.

I am looking forward to hear back from you.

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Question

Sorry for the late reply but I was on vacation last week. I had tried the Ab at a 1000 dilution. Could you please send as replacement the ab97130

Thanks

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Answer

Thank you foryour email.

I am sorryfor theinconvenience this has caused. As requested, I have issued a free of charge replacement of one unit of ab97130 with the order number 1076961. The vial should arrive tomorrow at your place. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

In order to complete our records, can you please confirm the following in regards to the ab7111:
The first vial was contaminated. The second vial had no contamination. Both vials have been tested at 1/1000 dilution and have not provided any signal.
Thankyou for confirming this. I am looking forward to your reply.

I wish you the best of luck with your research.

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Question

phone call reporting vial contamination

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Answer

Thank you for having contacted us with the contamination problem with a vial of ab7111. I would like to apologize again for this error.

We have sent you a replacement vial, which should have arrived last Wednesday. I am writing you now, to see whetherit arrived well and to be reassured that you are satisfied with the new vial.

Thank you for a short feedback. I am looking forward to hear back from you.

I wish you all the best of success for your research.

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Question

contamination in vial - big fluffy cream colored thing, probably fungus

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Answer

Thank you for your phone call. Unfortunately the vial we had ready to ship for you was also contaminated upon check. We are receiving however today the new stock from the our US office. I can unfortunately not guartantee that we can ship it out today still. I am very sorry. I will update you as soon as I know more.

The order number is xx and will be shipped as soon as possible. The new vial will be from batch xx and will be also in an amber vial. We changed the vials recently, just to be sure that long exposure to light would not damage the conjugate.

Please do not hesitate to contact me should you have any question. I thank you for your cooperation and understanding.

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