Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
Key features and details
- Rabbit Anti-Sheep IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
製品の概要
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製品名
Rabbit Anti-Sheep IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Sheep -
アプリケーション
適用あり: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
免疫原
Sheep IgG whole molecule
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Horseradish Peroxidase (HRP).
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研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
Our Abpromise guarantee covers the use of ab6747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/4000. PubMed: 15319485 | |
| ELISA | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/500 - 1/2500. | |
| IHC-Fr | Use at an assay dependent concentration. |
画像
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Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (27)
ab6747 は 27 報の論文で使用されています。
- Xiao Z et al. Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. Oncol Rep 41:2667-2678 (2019). PubMed: 30896884
- Wiese M et al. Citrullination of HP1? chromodomain affects association with chromatin. Epigenetics Chromatin 12:21 (2019). PubMed: 30940194
- Ortega-Vargas S et al. Moderate protection is induced by a chimeric protein composed of leucine aminopeptidase and cathepsin L1 against Fasciola hepatica challenge in sheep. Vaccine 37:3234-3240 (2019). PubMed: 31036453
- Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390
- Ye J et al. Low-Concentration PTX And RSL3 Inhibits Tumor Cell Growth Synergistically By Inducing Ferroptosis In Mutant p53 Hypopharyngeal Squamous Carcinoma. Cancer Manag Res 11:9783-9792 (2019). PubMed: 31819616
