Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

Reviews (1)Q&A (2)References (27)

Key features and details

  • Rabbit Anti-Sheep IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr

製品の概要

  • 製品名

    Rabbit Anti-Sheep IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Sheep

  • アプリケーション

    適用あり: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details

  • 免疫原

    Sheep IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項

    Horseradish Peroxidase (HRP).

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. PubMed: 15319485
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-Fr Use at an assay dependent concentration.

画像

  • Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).

    Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (27)

ab6747 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6747 は 27 報の論文で使用されています。

  • Xiao Z  et al. Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. Oncol Rep 41:2667-2678 (2019). PubMed: 30896884
  • Wiese M  et al. Citrullination of HP1? chromodomain affects association with chromatin. Epigenetics Chromatin 12:21 (2019). PubMed: 30940194
  • Ortega-Vargas S  et al. Moderate protection is induced by a chimeric protein composed of leucine aminopeptidase and cathepsin L1 against Fasciola hepatica challenge in sheep. Vaccine 37:3234-3240 (2019). PubMed: 31036453
  • Panier S  et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390
  • Ye J  et al. Low-Concentration PTX And RSL3 Inhibits Tumor Cell Growth Synergistically By Inducing Ferroptosis In Mutant p53 Hypopharyngeal Squamous Carcinoma. Cancer Manag Res 11:9783-9792 (2019). PubMed: 31819616
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

A good 2nd antibody for WB

 Excellent
Abreviews
Application
Western blot
This is a good 2nd antibody for WB assay.

Abcam user community

Verified customer

投稿 Jun 24 2020

Question

Estimado/a Sr/Sra:

Me pongo en contacto con usted porque estoy interesada en tener los certificados de análisis de los siguientes productos que hemos adquirido de Abcam:

-hTF Sheep polyclonal Ab (Ab61372), batch: XXXXX
-anti- sheep Ab (Ab6747), batch: XXXXX

Por favor, le rogaría me lo pudiese proporcionar lo antes posible.

Un cordial saludo

María

Read More
Answer

Gracias por contactarnos.

Lamentablemente no enviamos Certificados de Análisis de ninguno de nuestros productos.

Lo que si podemos enviaros, y los adjunto a este mail, son Certificados de Conformidad.

Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

Read More

Question

Dear Sir



The customer would like to get the certification of ab53586 .lot.GR10254 and ab6747 lot 948846.



Pleases kindly send to me.





Best regards

Read More
Answer

Thank you for your inquiry.

Please find attached the Certificates of Compliance for ab6747 and ab53586.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com