Rabbit Anti-Sheep IgG H&L (Biotin) (ab6746)
Key features and details
- Rabbit Anti-Sheep IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
製品の概要
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製品名
Rabbit Anti-Sheep IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Sheep -
アプリケーション
適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Sheep IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab6746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/5000. | |
| ELISA | 1/140000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/500 - 1/3000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (4)
ab6746 は 4 報の論文で使用されています。
- Lu R et al. Inhibition of CD133 Overcomes Cisplatin Resistance Through Inhibiting PI3K/AKT/mTOR Signaling Pathway and Autophagy in CD133-Positive Gastric Cancer Cells. Technol Cancer Res Treat 18:1533033819864311 (2019). PubMed: 31405336
- Thibault K et al. BDNF-dependent plasticity induced by peripheral inflammation in the primary sensory and the cingulate cortex triggers cold allodynia and reveals a major role for endogenous BDNF as a tuner of the affective aspect of pain. J Neurosci 34:14739-51 (2014). IHC ; Rat . PubMed: 25355226
- Periz J et al. Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid. Proc Natl Acad Sci U S A 110:12042-7 (2013). PubMed: 23818620
- Lindersson E et al. p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies. J Biol Chem 280:5703-15 (2005). PubMed: 15590652
