Rabbit Anti-Rat IgG H&L (Texas Red ®) (ab6732)
Key features and details
- Rabbit Anti-Rat IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
製品の概要
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製品名
Rabbit Anti-Rat IgG H&L (Texas Red ®)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Rat -
アプリケーション
適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
免疫原
Rat IgG whole molecule
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標識
Texas Red ®. Ex: 596nm, Em: 620nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG -
ポリ/モノ
ポリクローナル -
研究分野
関連製品
アプリケーション
Our Abpromise guarantee covers the use of ab6732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. |
画像
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Rat IgG H&L (Texas Red ®) (ab6732)Image from Sun Y et al., J Biol Chem. 2012 Feb 3;287(6):4275-87. Epub 2011 Dec 13. Fig 2.; doi: 10.1074/jbc.M111.280016; February 3, 2012 The Journal of Biological Chemistry, 287, 4275-4287.Immunofluorescence analysis of mouse fibroblasts, stained with a rat anti-mouse Lamp1 primary antibody and detected with ab6732 at 1/100 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (4)
ab6732 は 4 報の論文で使用されています。
- Leclaire MD et al. Lipofuscin-dependent stimulation of microglial cells. Graefes Arch Clin Exp Ophthalmol N/A:N/A (2019). PubMed: 30693383
- Su N et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425
- Sun Y et al. Ex Vivo and in Vivo Effects of Isofagomine on Acid ß-Glucosidase Variants and Substrate Levels in Gaucher Disease. J Biol Chem 287:4275-87 (2012). ICC/IF . PubMed: 22167193
- Teranishi N et al. Identification of neovasculature using nestin in colorectal cancer. Int J Oncol 30:593-603 (2007). PubMed: 17273760
