Rabbit Anti-Rat IgG H&L (Texas Red ®) (ab6732)
Key features and details
- Rabbit Anti-Rat IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
製品の概要
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製品名
Rabbit Anti-Rat IgG H&L (Texas Red ®)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Rat -
アプリケーション
適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
免疫原
Rat IgG whole molecule
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標識
Texas Red ®. Ex: 596nm, Em: 620nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項（標識）
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6732の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| ICC/IF |
1/1000 - 1/5000.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 特記事項 |
|---|
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
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ELISA
1/10000 - 1/50000. |
画像
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Rat IgG H&L (Texas Red ®) (ab6732)Image from Sun Y et al., J Biol Chem. 2012 Feb 3;287(6):4275-87. Epub 2011 Dec 13. Fig 2.; doi: 10.1074/jbc.M111.280016; February 3, 2012 The Journal of Biological Chemistry, 287, 4275-4287.Immunofluorescence analysis of mouse fibroblasts, stained with a rat anti-mouse Lamp1 primary antibody and detected with ab6732 at 1/100 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab6732 は 4 報の論文で使用されています。
- Leclaire MD et al. Lipofuscin-dependent stimulation of microglial cells. Graefes Arch Clin Exp Ophthalmol N/A:N/A (2019). PubMed: 30693383
- Su N et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425
- Sun Y et al. Ex Vivo and in Vivo Effects of Isofagomine on Acid ß-Glucosidase Variants and Substrate Levels in Gaucher Disease. J Biol Chem 287:4275-87 (2012). ICC/IF . PubMed: 22167193
- Teranishi N et al. Identification of neovasculature using nestin in colorectal cancer. Int J Oncol 30:593-603 (2007). PubMed: 17273760