Rabbit Anti-Rat IgG H&L (HRP) (ab102172)
Key features and details
- Rabbit Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
製品の概要
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製品名
Rabbit Anti-Rat IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rabbit -
ターゲット生物種
Rat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: IHC-P, ICC, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Part of the AbExcel range. -
研究分野
関連製品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/5000.
|
|
| ICC |
1/200 - 1/5000.
|
|
| ELISA |
1/10000 - 1/100000. (as primary)
|
|
| WB |
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/5000. |
|
ICC
1/200 - 1/5000. |
|
ELISA
1/10000 - 1/100000. (as primary) |
|
WB
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000). |
画像
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Western blot - Rabbit Anti-Rat IgG H&L (HRP) (ab102172)Lane 1 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
Lanes 2-6 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/2000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/10000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lane 5 : Goat polyclonal to RUNX2 (ab102712) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lane 6 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab102172 は 3 報の論文で使用されています。
- Bochnakian A et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
- Fung L et al. miR-128 Restriction of LINE-1 (L1) Retrotransposition Is Dependent on Targeting hnRNPA1 mRNA. Int J Mol Sci 20:N/A (2019). PubMed: 31010097
- Idica A et al. MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1. J Biol Chem 292:20494-20508 (2017). PubMed: 28974576