Rabbit Anti-Rat IgG H&L (FITC) preadsorbed (ab7100)

Submit a review Q&A (3)

Key features and details

  • Rabbit Anti-Rat IgG H&L (FITC) preadsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA

製品の概要

  • 製品名

    Rabbit Anti-Rat IgG H&L (FITC) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Rat

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Full length native Rat IgG (purified).

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7100の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
IHC-Fr
Immunomicroscopy
Flow Cyt
ELISA
特記事項
追加情報
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab7100 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7100 は論文での使用が確認できていません。

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Thank you. Do you have any idea when this will arrive?

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Answer

Thank you very much for your reply.

I've looked at the package'stracking information, and it arrived on September 20 at 9:34 EST. You can track the package on FedEx.com with tracking number: ***. It was signed for by ***- does that help you track it down?

Please let me know if you can find thepackage with this information, or if you need any furtherassistance and I'll be happy to help.

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Question

For my experiments, I am using mouse osteoblasts which express EGFR. Also, I use 2% mouse serum for blocking and a dilution of 1:100 and 1:400. Under these conditions, the secondary antibody alone is giving a positive signal. If possible, I would like to get a replacement with AB6115 as noted below. My PO number is shown below.


Thank you for the technical support,

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Answer

Thank you very much for your reply.

It does sound like the secondary is reacting with the IgG's in the mouse serum. I'm sending a free of charge vial of ab6115 on the order *** which should arrive tomorrow.

Please keep me updated about the results using this secondary, and if there is anything else that we can do for you please let me know.

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Question

Inquiry: I am trying to use ab231 and ab7100 for detecting EGFR in mouse by flow cytometry. However, the secondary antibody is giving a high background signal. I am assuming this is because it reacts with mouse. Would you agree with this interpretation and if so is there another secondary antibody that you would recommend? I had previously contacted customer service and they suggested that I use ab7100. Thanks,

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Answer

Thank you very much for contacting us with your question.

It is possible that the secondary antibody is causing some background signal, as ab7100 has been pre-adsorbed only against human serum proteins and not mouse serum proteins. Have you determined that the secondary antibody is causing the background due to signal in a "no primary" or isotype control sample? What dilution of ab7100 are you using, and what kind of mouse cells are you analyzing? What kind of blocking solution do you use?

If you know that the background is coming from the secondary antibody, I'd suggest using a secondary antibody that has been pre-adsorbed against mouse serum proteins. Ab6115 is a goat anti-rat antibody that has been pre-adsorbed against mouse. I'd be happy to send this as a replacement for ab7100 if it is not working as it should be. Please send me your order or PO number if you would like to receive this replacement.

I look forward to hearing from you. If there is anything else that we can do for you, please let me know and I'll be happy to help.

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