Rabbit Anti-Rat IgG H&L (FITC) (ab6730)

Reviews (1)Q&A (2)References (11)
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)

    Key features and details

    • Rabbit Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    製品の概要

    • 製品名

      Rabbit Anti-Rat IgG H&L (FITC)
      IgG 二次抗体 製品一覧

    • 由来種

      Rabbit

    • ターゲット生物種

      Rat

    • アプリケーション

      適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • 免疫原

      Rat whole molecule

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 精製度

      Affinity purified

    • 特記事項（精製）

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 特記事項（標識）

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • ポリ/モノ

      ポリクローナル

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6730の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF (1)
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    特記事項
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    画像

    • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
      Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
      Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (11)

    ab6730 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6730 は 11 報の論文で使用されています。

    • Zhu L  et al. Ampelopsin-sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncol Lett 18:189-196 (2019). PubMed: 31289488
    • Xu Y  et al. Differentiation of CD45-/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43:1128-1138 (2019). PubMed: 30628669
    • Kneppers A  et al. A novel in vitro model for the assessment of postnatal myonuclear accretion. Skelet Muscle 8:4 (2018). PubMed: 29444710
    • Gholinejad M  et al. Adenosine decreases oxidative stress and protects H2O2-treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8:439-446 (2018). PubMed: 29732147
    • Wan B  et al. Topotecan alleviates ventilator-induced lung injury via NF-?B pathway inhibition. Cytokine N/A:N/A (2018). PubMed: 29803659
    View all Publications for this product

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Secondary ab

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    It works good with abcam as well as other vendors antibodies

    Abcam user community

    Verified customer

    投稿 Sep 05 2014

    Question

    I'm actually looking for an isotype control that would fit with the ab15684(smooth muscle cells antibody HM19/2). Ideally, I would like to use a rat IgM.Do you have any suggestions? Would a rat IgG be a good isotype for immunofluorescence with that antibody? The same question can be apply for the secondary antibody. The technical data sheet suggest ab6730 which is a rabbit polyclonal to rat IgG coupled to a FITC molecule. Is it a good choice?

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    Answer

    Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view Cy5 might be brighter than FITC and an amplification with HRP or biotin can also enhance greatly your signal if you suspect low abundance of the protein in your samples). You have not mentioned the source of your cells, but please remember the antibody is a rat monoclonal so it cannot be used on rat cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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    Question

    Hi, I am using Rat monoclonal (YOL1/34) to alpha Tubulin (ab9267), dispatched on: 6-Aug-2003 and Rabbit polyclonal to Rat IgG H&L (FITC) (ab6730, dispatched on: 6-Aug-2003. I would like to visualize Plant (Arabidopsis) alpha tubulin. Did any of your customers already try these antibodies in planta?

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    Answer

    We have had no feedback from customers that may have used these antibodies in plant.

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