Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

Submit a review Q&A (1)References (10)
Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

    Key features and details

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: ICC/IF, IHC-P

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
      IgG + IgM + IgA 二次抗体 製品一覧

    • 由来種

      Rabbit

    • ターゲット生物種

      Mouse

    • 特異性

      Antibodies were raised in rabbit to mouse IgG, IgM and IgA

    • アプリケーション

      適用あり: ICC/IF, IHC-Pmore details

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.50
      Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.

    • 特記事項（標識）

      The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.

    • ポリ/モノ

      ポリクローナル

    • 特記事項

      F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab8517の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    Use at an assay dependent dilution. PubMed: 15483223
    IHC-P
    Use at an assay dependent dilution.
    特記事項
    ICC/IF
    Use at an assay dependent dilution. PubMed: 15483223
    IHC-P
    Use at an assay dependent dilution.

    画像

    • Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (10)

    ab8517 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8517 は 10 報の論文で使用されています。

    • Zhao M  et al. The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells. Cell Prolif 53:e12758 (2020). PubMed: 31922317
    • Akyash F  et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod Biomed (Yazd) 17:891-906 (2019). PubMed: 31970311
    • Kolodziej M  et al. First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland. Ann Agric Environ Med 25:368-373 (2018). PubMed: 29936795
    • Ahn J  et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . PubMed: 26432097
    • Michalski A  et al. Surveillance of hantaviruses in Poland: a study of animal reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis 14:514-22 (2014). PubMed: 24902039
    • Guo Z  et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739
    • Tang L  et al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 226:832-42 (2011). IHC-P . PubMed: 20857425
    • Huang CY  et al. Plasticity of stem cells derived from adult periodontal ligament. Regen Med 4:809-21 (2009). PubMed: 19903001
    • Cai L  et al. Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy. J Am Coll Cardiol 48:1688-97 (2006). PubMed: 17045908
    • Pfister-Genskow M  et al. Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer: improper reprogramming of genes required for development. Biol Reprod 72:546-55 (2005). ICC/IF ; Mouse . PubMed: 15483223

    レビューと Q&A

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    Question

    compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

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    Answer

    When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM.

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