Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (ab7075)

Submit a review Q&A (3)References (2)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

製品の概要

  • 製品名

    Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Rabbit

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 吸着処理血清


    Human more details

  • 免疫原

    Full length native Mouse IgG (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
ELISA 1/5000 - 1/20000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/500 - 1/2500.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab7075 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7075 は 2 報の論文で使用されています。

  • Whelan DR  et al. Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks. Nat Commun 9:3882 (2018). PubMed: 30250272
  • Gupta V  et al. Inhaled PLGA particles of prostaglandin E1 ameliorate symptoms and progression of pulmonary hypertension at a reduced dosing frequency. Mol Pharm 10:1655-67 (2013). WB . PubMed: 23485062

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Question

Dear Sir or Madam,

We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

Could you send me a quote for the antibodies listed below?


Anti-6X His tag® antibody [3H2201] (ab15149)

Anti-6X His tag® antibody (ab125262)

Anti-6X His tag® antibody (Agarose) (ab90663)

Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
Anti-Hepatitis B Virus X antigen antibody (ab39716)

Anti-p53 antibody [PAb 240] (ab26)

I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

Best wishes

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Answer

Sorry for the delay in getting back to you. I now have further information to share with you.

I have checked into whether anything is know about the epitope of the anti-Hepatitis B Virus X antigen antibodies ab235, ab2741 and ab39716. I can only provide further information for ab235 additionally to the information provided on the datasheet of the antibodies. The epitope recognised by ab235 is thought to recognise an epitope of the following sequence:

DVLCLRPVGASESR.

I was incorrect in my last email to say that ab235 had not been tested in Western blotting. It have been tested but we have had mixed reports from customers so we would not recommend buying this antibody with that application in mind.

I have also looked into the anti-Hepatitis B Virus Core Antigen antibodies we have and if anything is known of the conformation of the epitope is known. Unfortunately, I could not find any antibodies where this information had been established and most are able to recognise both the denatured and native form of the protein.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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Question

Dear Sir or Madam,

We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

Could you send me a quote for the antibodies listed below?


Anti-6X His tag® antibody [3H2201] (ab15149)

Anti-6X His tag® antibody (ab125262)

Anti-6X His tag® antibody (Agarose) (ab90663)

Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
Anti-Hepatitis B Virus X antigen antibody (ab39716)

Anti-p53 antibody [PAb 240] (ab26)

I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

Best wishes

Read More
Answer

Thank you for contacting us and your interest in our products.

The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other questions:

The Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) should work well with ab15149, ab2741, ab235 and ab26. You would need a different secondary antibody for all the other antibodies mentioned as they are raised in rabbit. You may wantto consider the Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080). Please bear in mind that we have as yet not tested the anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235) in Western blotting or immunoprecipitation. This does not mean that it would not work, simply that we have not tested it ourselves as yet and would therefore not he able to guarantee its performance in these applications.

We have some good resources in setting up both immunoprecipitation and Western blotting experiments from the following link:

https://docs.abcam.com/pdf/misc/abcam-protocols-book-2010.pdf

Some further troubleshooting tips can also be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=11382

If you would like any help with this please do let us know.

I will check if any further information is know about the epitopes recognised by the anti-HBx antibodies mentioned and will get back to you with this information as soon as I have it. I will also look into your final question ifwe have any conformation specific antibodies against HBV core protein (HBc).

As you will be setting up these experiments from scratch, you may be interested in looking at our Western blotting range:

https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot

The link above will take you not only to our Optiblot range of gels which provide superior durability and ease of use compared to many standard gels, but also the PVDF and nitrocellulose membranes and Ecl Detection kits we have available.

If you would like any further information on these products please do let us know.

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Question

Inquiry: Dear, Does this product cross reacts with IgG from other species (e.g. human, rat, ...)? If so do you have another one that does react with mouse IgG but not with human IgG?

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Answer

Thank you for contacting us.
As this antibody has only been purified against it immunogen, it may cross- react with other species.
I have picked out three pre-adsorbed secondary antibodies, which show minimal cross-reactivity to human IgG:
https://www.abcam.com/index.html?datasheet=7075 https://www.abcam.com/index.html?datasheet=7075.
https://www.abcam.com/index.html?datasheet=98780 https://www.abcam.com/index.html?datasheet=98780.
https://www.abcam.com/index.html?datasheet=98808 https://www.abcam.com/index.html?datasheet=98808.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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