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  1. Link

    rabbit-mouse-igg-hl-hrp-ab6728.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  • Datasheet
  • SDS
Reviews (10)Q&A (31)References (677)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 647 Alkaline Phosphatase Biotin FITC Texas Red ® TRITC Unconjugated Unconjugated Unconjugated

こちらの製品もご検討ください

Western blot
Nitrocellulose Transfer Membrane- 0.22 µm (ab133413)
一次抗体
Product image
Anti-Smad2 + Smad3 antibody [EPR19557] - BSA and Azide free (ab236030)

関連製品

製品の概要

  • 製品名

    Rabbit Anti-Mouse IgG H&L (HRP)
    IgG 二次抗体 製品一覧
  • 由来種

    Rabbit
  • ターゲット生物種

    Mouse
  • アプリケーション

    適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • 特記事項(標識)

    Horseradish Peroxidase (HRP)
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

関連製品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6728の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use at an assay dependent dilution.
ELISA
1/1000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
WB (10)
1/2000 - 1/10000. PubMed: 17200442
特記事項
Dot blot
Use at an assay dependent dilution.
ELISA
1/1000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
WB
1/2000 - 1/10000. PubMed: 17200442

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.

    Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

    Black arrows indicate double-positive cells.

    Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

    Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)This image is courtesy of an anonymous Abreview.

    ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (677)

ab6728 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6728 は 677 報の論文で使用されています。

  • Zhou C  et al. Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment. Cell Death Differ 28:715-729 (2021). PubMed: 32929219
  • Song T  et al. Zearalenone exposure affects the Wnt/ß-catenin signaling pathway and related genes of porcine endometrial epithelial cells in vitro. Anim Biosci 34:993-1005 (2021). PubMed: 32898953
  • Qiu J  et al. Role of TGF-ß1-mediated epithelial-mesenchymal transition in the pathogenesis of tympanosclerosis. Exp Ther Med 21:6 (2021). PubMed: 33235615
  • Liu X  et al. The AMPK-mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis. Exp Ther Med 21:21 (2021). PubMed: 33235630
  • Kashkoulinejad-Kouhi T  et al. Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3? co-knockdown. Oncol Rep 45:665-679 (2021). PubMed: 33416155
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 41 Abreviews or Q&A

secondary antibody ab6728

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
dilution buffer: 5% non-fat milk in PBS-Tween
dilution: 1/20000
incubation time: 1hour
exposure time: 30sec
Figure legend:
Western blot for cyclin B2 and GAPDH. HeLa Kyoto cells transfected with 50nM siRNA against HURP protein(lane 1) or 50nM non-targeting siRNA(lane 2).

MR. Christos Efstathiou

Verified customer

投稿 Mar 09 2022

Amazing WB Secondary

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
loaded 20ug human cell lysate
reduced and denatured conditions
primary antibody was a mouse-anti-human & incubated for 16hr at 4degrees in 5% milk
secondary (ab6728) incubated for 1hr, at 1:5000 in 5% milk at 20degrees

from left to right:
Lane 1 = human cell lysate
Lane 2 = human cell lysate
Ladder

Abcam user community

Verified customer

投稿 Mar 31 2022

Works Perfect

Excellent
Abreviews
Abreviews
Application
Western blot
Works great as described.

Abcam user community

Verified customer

投稿 Jun 18 2021

EXCELLENT SECONDARY ANTIBODY for WB

Excellent
Abreviews
Abreviews
Application
Western blot
EXCELLENT SECONDARY ANTIBODY for WB

MRS. Kyunga Kwak

Verified customer

投稿 May 07 2020

Very sensitive and specific secondary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).

Figure legend
Western blot image of actin bands. HCT116 cells transfected with non-targeting siRNA (lane #1) or CDK4/6 siRNA (lane #2).

Abcam user community

Verified customer

投稿 Oct 02 2018

Immunoblot detection of B-3-Tubulin

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
20 μg of micro tubular protein from SH-SY5y cells were used to detect B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728 - 1: 2000 dilution for 1h).

Abcam user community

Verified customer

投稿 Feb 28 2018

Excellent 2nd antibody for WB

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)

MR. Baptiste Jude

Verified customer

投稿 Oct 10 2016

This is a good 2nd antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.

Abcam user community

Verified customer

投稿 May 30 2014

The product is good

Excellent
Abreviews
Abreviews
Application
Western blot
When I perform WB, that is very sensitive. making band is clearly

Abcam user community

Verified customer

投稿 Jan 23 2014

good for mouse primary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
strong detection

Abcam user community

Verified customer

投稿 Jul 11 2013

1-10 of 41 Abreviews or Q&A

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